Gan Weidong, Zhou Ming, Xiang Zou, Han Xiaodong, Li Dongmei
Department of Urology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Medical School, Nanjing University, Nanjing 210008, China.
Immunology and Reproductive Biology Laboratory & State Key Laboratory of Analytical Chemistry for Life Science, Medical School, Nanjing University, Nanjing 210093, China.
Int J Environ Res Public Health. 2015 Apr 14;12(4):4141-55. doi: 10.3390/ijerph120404141.
The xenoestrogens nonylphenol (NP) and bisphenol A (BPA) are regarded as endocrine disrupting chemicals (EDCs) which have widespread occurrence in our daily life. In the present study, the purpose was to analyze the combined effects of NP and BPA on the human prostate epithelial cell line RWPE-1 using two mathematical models based on the Loewe additivity (LA) theory and the Bliss independence (BI) theory. RWPE-1 cells were treated with NP (0.01-100 µM) and BPA (1-5000 µM) in either a single or a combined format. A cell viability assay and lactate dehydrogenase (LDH) leakage rate assay were employed as endpoints. As predicted by the two models and based on the cell viability assay, significant synergism between NP and BPA were observed. However, based on the LDH assay, the trends were reversed. Given that environmental contaminants are frequently encountered simultaneously, these data indicated that there were potential interactions between NP and BPA, and the combined effects of the chemical mixture might be stronger than the additive values of individual chemicals combined, which should be taken into consideration for the risk assessment of EDCs.
异雌激素壬基酚（NP）和双酚A（BPA）被视为内分泌干扰化学物质（EDC），在我们的日常生活中广泛存在。在本研究中，目的是使用基于洛维加和性（LA）理论和布利斯独立性（BI）理论的两个数学模型，分析NP和BPA对人前列腺上皮细胞系RWPE - 1的联合作用。RWPE - 1细胞用NP（0.01 - 100 μM）和BPA（1 - 5000 μM）以单一或联合形式处理。采用细胞活力测定和乳酸脱氢酶（LDH）泄漏率测定作为终点指标。正如两个模型所预测的，并基于细胞活力测定，观察到NP和BPA之间存在显著的协同作用。然而，基于LDH测定，趋势则相反。鉴于环境污染物经常同时出现，这些数据表明NP和BPA之间存在潜在的相互作用，并且化学混合物的联合作用可能比单个化学物质相加的值更强，这在EDC的风险评估中应予以考虑。
