Azab Hassan A, Khairy Gasser M, Kamel Rasha M
Chemistry Department, Faculty of Science, Suez Canal University, 41522 Ismailia, Egypt.
Chemistry Department, Faculty of Science, Suez Canal University, 41522 Ismailia, Egypt; Chemistry Department, Faculty of Science and Arts, Aljouf University, P.O. Box #2014, Skaka 41421, Saudi Arabia.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Sep 5;148:114-24. doi: 10.1016/j.saa.2015.03.098. Epub 2015 Apr 4.
This work describes the application of time resolved fluorescence in microtiter plates for investigating the interactions of europium-allyl-3-carboxycoumarin with pesticides chlorpyrifos, endosulfan and crotoxyphos. Stern-Volmer studies at different temperatures for chlorpyrifos and crotoxyphos shows dynamic and static quenching mechanisms respectively. Direct methods for the determination of the pesticides under investigation have been developed using the luminescence variations of the probe in solution. The detection limits are 6.53, 0.004, 3.72 μmol/L for chlorpyrifos, endosulfan, and crotoxyphos, respectively. The binding constants and thermodynamic parameters of the pesticides with probe were evaluated. A thermodynamic analysis showed that the reaction is spontaneous with negative ΔG. Effect of some relevant interferents on the detection of pesticides has been investigated. The new method was applied to the determination of the pesticides in different types of water samples (tap, mineral, and waste water).
这项工作描述了时间分辨荧光在微量滴定板中的应用，用于研究铕-烯丙基-3-羧基香豆素与农药毒死蜱、硫丹和巴丹的相互作用。在不同温度下对毒死蜱和巴丹进行的斯特恩-沃尔默研究分别显示了动态和静态猝灭机制。利用溶液中探针的发光变化，开发了测定所研究农药的直接方法。毒死蜱、硫丹和巴丹的检测限分别为6.53、0.004、3.72μmol/L。评估了农药与探针的结合常数和热力学参数。热力学分析表明，该反应是自发的，ΔG为负。研究了一些相关干扰物对农药检测的影响。该新方法应用于不同类型水样（自来水、矿泉水和废水）中农药的测定。
