一种通过固相萃取和柱前衍生化测定猪肉样品中莱克多巴胺残留量的高效液相色谱法的建立与验证
Development and validation of a high-performance liquid chromatography method for determination of ractopamine residue in pork samples by solid phase extraction and pre-column derivatization.
作者信息
Ding Guanglong, Li Deguang, Qin Jiao, Zhu Juanli, Wang Baitao, Geng Qianqian, Guo Mingcheng, Punyapitak Darunee, Cao Yongsong
机构信息
College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
出版信息
Meat Sci. 2015 Aug;106:55-60. doi: 10.1016/j.meatsci.2015.03.016. Epub 2015 Mar 27.
Ractopamine (RAC) has been approved as a feed additive for swine, cattle or turkey, and is likely to have residue in edible animal products and may pose a potential risk for consumer health. Therefore, it is essential to establish a method to detect the residue of RAC in animal products. This work presents a rapid and sensitive HPLC method for the determination of RAC in pork samples with pre-column derivatization. The RAC derivative was separated on a kromasil C18 column and detected at 284nm with a UV detector. The detection capability (CCβ) was 0.078μgg(-1) and the linearity was established over the concentration range of 0.15-100.0μgg(-1). The overall mean recovery in spike range of 0.2μgg(-1) to 100μgg(-1) was 89.9% with the overall mean relative standard deviation of 4.1%. This method can be used for the quantification of RAC in pork samples and help to establish adequate monitoring of the residue of RAC.
莱克多巴胺（RAC）已被批准作为猪、牛或火鸡的饲料添加剂，很可能在食用动物产品中残留，并可能对消费者健康构成潜在风险。因此，建立一种检测动物产品中莱克多巴胺残留的方法至关重要。本研究建立了一种快速灵敏的高效液相色谱法，采用柱前衍生化法测定猪肉样品中的莱克多巴胺。莱克多巴胺衍生物在kromasil C18柱上分离，用紫外检测器在284nm处检测。检测限（CCβ）为0.078μg g⁻¹，在0.15 - 100.0μg g⁻¹浓度范围内建立了线性关系。在0.2μg g⁻¹至100μg g⁻¹加标范围内，总体平均回收率为89.9%，总体平均相对标准偏差为4.1%。该方法可用于猪肉样品中莱克多巴胺的定量分析，有助于建立对莱克多巴胺残留的充分监测。
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