Srikanth Koigoora, Mahajan Amit, Pereira Eduarda, Duarte Armando Costa, Venkateswara Rao Janapala
CESAM-Centre for Environmental and Marine Studies & Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Portugal.
Department of Bioresource Sciences, Andong National University, Andong, Republic of Korea.
J Appl Toxicol. 2015 Oct;35(10):1133-40. doi: 10.1002/jat.3142. Epub 2015 Apr 15.
Aluminium oxide nanoparticles (Al2 O3 NPs) are increasingly used in diverse applications that has raised concern about their safety. Recent studies suggested that Al2 O3 NPs induced oxidative stress may be the cause of toxicity in algae, Ceriodaphnia dubia, Caenorhabditis elegans and Danio rerio. However, there is paucity on the toxicity of Al2 O3 NPs on fish cell lines. The current study was aimed to investigate Al2 O3 NPs induced cytotoxicity, oxidative stress and morphological abnormality of Chinnok salmon cells (CHSE-214). A dose-dependent decline in cell viability was observed in CHSE-214 cells exposed to Al2 O3 NPs. Oxidative stress induced by Al2 O3 NPs in CHSE-214 cells has resulted in the significant reduction of superoxide dismutase, catalase and glutathione in a dose-dependent manner. However, a significant increase in glutathione sulfo-transferase and lipid peroxidation was observed in CHSE-214 cells exposed to Al2 O3 NPs in a dose-dependent manner. Significant morphological changes in CHSE-214 cells were observed when exposed to Al2 O3 NPs at 6, 12 and 24 h. The cells started to detach and appear spherical at 6 h followed by loss of cellular contents resulting in the shrinking of the cells. At 24 h, the cells started to disintegrate and resulted in cell death. Our data demonstrate that Al2 O3 NPs induce cytotoxicity and oxidative stress in a dose-dependent manner in CHSE-214 cells. Thus, our current work may serve as a base-line study for future evaluation of toxicity studies using CHSE-214 cells.
氧化铝纳米颗粒（Al2O3 NPs）在各种应用中的使用日益增加，这引发了人们对其安全性的担忧。最近的研究表明，Al2O3 NPs诱导的氧化应激可能是藻类、大型蚤、秀丽隐杆线虫和斑马鱼中毒性的原因。然而，关于Al2O3 NPs对鱼类细胞系毒性的研究较少。本研究旨在探讨Al2O3 NPs对奇努克鲑鱼细胞（CHSE-214）的细胞毒性、氧化应激和形态异常。在暴露于Al2O3 NPs的CHSE-214细胞中观察到细胞活力呈剂量依赖性下降。Al2O3 NPs在CHSE-214细胞中诱导的氧化应激导致超氧化物歧化酶、过氧化氢酶和谷胱甘肽以剂量依赖性方式显著降低。然而，在暴露于Al2O3 NPs的CHSE-214细胞中观察到谷胱甘肽硫转移酶和脂质过氧化以剂量依赖性方式显著增加。当在6、12和24小时暴露于Al2O3 NPs时，CHSE-214细胞出现明显的形态变化。细胞在6小时开始脱离并呈球形，随后细胞内容物丢失导致细胞收缩。在24小时时，细胞开始解体并导致细胞死亡。我们的数据表明，Al2O3 NPs在CHSE-214细胞中以剂量依赖性方式诱导细胞毒性和氧化应激。因此，我们目前的工作可作为未来使用CHSE-214细胞进行毒性研究评估的基线研究。
