Complete genome sequence of Lactococcus lactis subsp. lactis KLDS4. 0325, a bacterium newly isolated from Koumiss in Xinjiang, China.

OBJECTIVE

Aim to study the physiology and functionally important genes of Lactobacillus lactis KLDS4. 0325.

METHODS

We sequenced the complete genome of L. lactis KLDS4. 0325, drew the genomic map, and performed functional annotation and analysis in metabolism and probiotic.

RESULTS

L. lactis KLDS4. 0325 contains a chromosome of 2589261 bp, GC content is 35.4%, with 2662 predicted ORFs, of which 1310 are functionally classified. L. lactis KLDS4. 0325 can carry out hydrolysis of extracellular proteins effectively, has the potential to degrade bitter peptides and produce a series of peptides of inhibiting angiotensin converting enzyme. L. lactis KLDS4. 0325 has complete enzyme system for transamination pathway, which can catalyze relevant amino acids to flavor compounds. More key enzyme-coding genes involved in transport and metabolizing of sugars, and L-lactic acid synthesis, are exist in L. lactis KLDS4. 0325 genome. L. lactis KLDS4. 0325 has a set of more complete encoding genes in the biosynthetic pathways of folate and riboflavin. In addition, gene cluster for Lactococcin and 2 cold stress protein (cspD and cspE) were identified.

CONCLUSION

The presence of these genes encoding desirable traits provides the theoretical basis for the strain in industrial fermentation, and relevant further research.