Yang Xiaochun, Wang Yutang, Zhou Ying, Gao Xiaofeng, Li Bailiang, Huo Guicheng
Wei Sheng Wu Xue Bao. 2014 Dec 4;54(12):1406-18.
Aim to study the physiology and functionally important genes of Lactobacillus lactis KLDS4. 0325.
We sequenced the complete genome of L. lactis KLDS4. 0325, drew the genomic map, and performed functional annotation and analysis in metabolism and probiotic.
L. lactis KLDS4. 0325 contains a chromosome of 2589261 bp, GC content is 35.4%, with 2662 predicted ORFs, of which 1310 are functionally classified. L. lactis KLDS4. 0325 can carry out hydrolysis of extracellular proteins effectively, has the potential to degrade bitter peptides and produce a series of peptides of inhibiting angiotensin converting enzyme. L. lactis KLDS4. 0325 has complete enzyme system for transamination pathway, which can catalyze relevant amino acids to flavor compounds. More key enzyme-coding genes involved in transport and metabolizing of sugars, and L-lactic acid synthesis, are exist in L. lactis KLDS4. 0325 genome. L. lactis KLDS4. 0325 has a set of more complete encoding genes in the biosynthetic pathways of folate and riboflavin. In addition, gene cluster for Lactococcin and 2 cold stress protein (cspD and cspE) were identified.
The presence of these genes encoding desirable traits provides the theoretical basis for the strain in industrial fermentation, and relevant further research.
旨在研究乳酸乳球菌KLDS4.0325的生理及功能重要基因。
我们对乳酸乳球菌KLDS4.0325的全基因组进行了测序，绘制了基因组图谱，并在代谢和益生菌方面进行了功能注释与分析。
乳酸乳球菌KLDS4.0325含有一条2589261 bp的染色体，GC含量为35.4%，有2662个预测的开放阅读框（ORF），其中1310个进行了功能分类。乳酸乳球菌KLDS4.0325能够有效进行细胞外蛋白质水解，有降解苦味肽并产生一系列抑制血管紧张素转化酶肽段的潜力。乳酸乳球菌KLDS4.0325具有完整的转氨途径酶系统，可催化相关氨基酸生成风味化合物。在乳酸乳球菌KLDS4.0325基因组中存在更多参与糖转运与代谢以及L-乳酸合成的关键酶编码基因。乳酸乳球菌KLDS4.0325在叶酸和核黄素生物合成途径中有一套更完整的编码基因。此外，还鉴定出了乳链菌肽基因簇以及2个冷应激蛋白（cspD和cspE）。
这些编码理想性状的基因的存在为该菌株在工业发酵及相关进一步研究提供了理论依据。
