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[嫁接对铜胁迫下甜瓜(Cucumis melo)幼苗生理特性的影响]

[Effects of grafting on physiological characteristics of melon (Cucumis melo) seedlings under copper stress].

作者信息

Tan Ming-min, Zhang Xin-ying, Fu Qiu-shi, He Zhong-qun, Wang Huai-song

出版信息

Ying Yong Sheng Tai Xue Bao. 2014 Dec;25(12):3563-72.

Abstract

The effects of grafting on physiological characters of melon (Cucumis melo) seedlings under copper stress were investigated with Pumpkin Jingxinzhen No. 3 as stock and oriental melon IVF09 as scion. The results showed that the physiological characters of melon seedlings were inhibited significantly under copper stress. Compared with self-rooted seedlings, the biomass, the contents of photosynthetic pigment, glucose and fructose, the photosynthetic parameters, the activities of sucrose phosphate synthase, neutral invertase and acid invertase in the leaves of the grafted seedlings were increased significantly. The uptake of nutrients was improved with the contents of K, P, Na increased and the content of Cu decreased. When the concentration of Cu2+ stress was 800 micromol L(-1), the contents of Cu in the leaves and roots of the grafted seedlings were decreased by 31.3% and 15.2%, respectively. Endogenous hormone balance of seedlings was improved by grafting. In the grafted seedlings, the content of IAA and peroxidase activity were higher, whereas the contents of ABA, maleicdialdehyde, the activities of superoxide dismutase and catalase were lower than that in the control. It was concluded that the copper stress on the physiological characters of melon seedlings was relieved by grafting which improved the resistance of the grafted seedlings.

摘要

以南瓜京欣砧3号为砧木、东方甜瓜IVF09为接穗,研究了嫁接对铜胁迫下甜瓜(Cucumis melo)幼苗生理特性的影响。结果表明,铜胁迫显著抑制了甜瓜幼苗的生理特性。与自根苗相比,嫁接苗叶片的生物量、光合色素含量、葡萄糖和果糖含量、光合参数、蔗糖磷酸合酶、中性转化酶和酸性转化酶活性均显著提高。养分吸收得到改善,K、P、Na含量增加,Cu含量降低。当Cu2+胁迫浓度为800 μmol L(-1)时,嫁接苗叶片和根系中的Cu含量分别降低了31.3%和15.2%。嫁接改善了幼苗的内源激素平衡。在嫁接苗中,IAA含量和过氧化物酶活性较高,而ABA、丙二醛含量、超氧化物歧化酶和过氧化氢酶活性低于对照。研究得出结论,嫁接缓解了铜胁迫对甜瓜幼苗生理特性的影响,提高了嫁接苗的抗性。

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