Xie Hui, Wang Fengqin, Yin Shuangyao, Ren Tianbao, Song Andong
Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Science, Henan Agricultural University, Nongye Road No. 63, Zhengzhou, 450002, China.
Appl Biochem Biotechnol. 2015 May;176(2):613-24. doi: 10.1007/s12010-015-1599-x. Epub 2015 Apr 16.
In the field of biofuel ethanol, high-concentration- reducing sugars made from cellulosic materials lay the foundation for high-concentration ethanol fermentation. In this study, corn stover was pre-treated in a process combining chemical methods and steam explosion; the cellulosic hydrolyzed sugars obtained by fed-batch saccharification were then used as the carbon source for high-concentration ethanol fermentation. Saccharomyces cerevisiae 1308, Angel yeast, and Issatchenkia orientalis were shake-cultured with Pachysolen tannophilus P-01 for fermentation. Results implied that the ethanol yields from the three types of mixed strains were 4.85 g/100 mL, 4.57 g/100 mL, and 5.02 g/100 mL (separately) at yield rates of 91.6, 89.3, and 92.2%, respectively. Therefore, it was inferred that shock-fermentation using mixed strains achieved a higher ethanol yield at a greater rate in a shorter fermentation period. This study provided a theoretical basis and technical guidance for the fermentation of industrial high-concentrated cellulosic ethanol.
在生物燃料乙醇领域，由纤维素材料制成的高浓度还原糖为高浓度乙醇发酵奠定了基础。在本研究中，玉米秸秆采用化学方法和蒸汽爆破相结合的工艺进行预处理；然后将分批补料糖化获得的纤维素水解糖用作高浓度乙醇发酵 的碳源。酿酒酵母1308、安琪酵母和东方伊萨酵母与嗜鞣管囊酵母P - 01进行摇瓶培养用于发酵。结果表明，三种混合菌株的乙醇产量分别为4.85 g/100 mL、4.57 g/100 mL和5.02 g/100 mL，产率分别为91.6%、89.3%和92.2%。因此，推断混合菌株的冲击发酵在较短的发酵周期内以更高的速率实现了更高的乙醇产量。本研究为工业高浓度纤维素乙醇的发酵提供了理论依据和技术指导。
