胰岛素的致畸作用：对发育中的鸡胚的实验研究

Teratogenic effects of insulin: an experimental study on developing chick embryo.

作者信息

Bokariya Pradeep, Kothari Ruchi, Gujar Vijay K, Shende M R

机构信息

Department of Anatomy, Mahatma Gandhi Institute of Medical Sciences, Wardha, Maharashtra, India.

Department of Physiology, Mahatma Gandhi Institute of Medical Sciences, Wardha, Maharashtra, India.

出版信息

Indian J Pharmacol. 2015 Mar-Apr;47(2):212-4. doi: 10.4103/0253-7613.153433.

DOI:10.4103/0253-7613.153433

PMID:25878385

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4386134/

Abstract

OBJECTIVE

The objective was to observe the effect of insulin on chick embryos with reference to their growth and developmental defects.

MATERIALS AND METHODS

An experimental study was performed to assess any abnormal growth pattern caused by insulin. For this, two batches of 100 fertilized eggs were utilized. One batch of 50 was used as a control group and other as an experimental group. Insulin (2 IU) was injected on day 2 of incubation. Chicken eggs were dissected out on day 19 of incubation and were carefully observed for any congenital abnormalities. The embryos thus dissected out were subjected to measurement of crown-rump length (CRL), changes in weight of egg, volume of embryos were compared in two groups. The embryos were also examined for any congenital anomalies.

RESULTS

No major malformations were observed. Decrease in weight and CRLs was lower in the experimental group as compared to their control counterparts. Values for volume of the embryo were similar in two groups.

CONCLUSION

No obvious teratogenic effects are observed with insulin in the dose use for the study.

摘要

目的

观察胰岛素对鸡胚生长及发育缺陷的影响。

材料与方法

进行一项实验研究以评估胰岛素引起的任何异常生长模式。为此，使用了两批各100枚受精鸡蛋。一批50枚用作对照组，另一批用作实验组。在孵化第2天注射胰岛素（2国际单位）。在孵化第19天取出鸡蛋并仔细观察有无先天性异常。将如此取出的胚胎进行顶臀长度（CRL）测量，比较两组鸡蛋重量变化、胚胎体积。还对胚胎进行先天性异常检查。