白细胞介素-18基因-607A/C和-137C/G多态性与缺血性脑卒中易感性的研究
Investigation on the IL-18 -607A/C and -137C/G on the susceptibility of ischemic stroke.
作者信息
Shi Jin-He, Niu Li-Dan, Chen Xi-Yan, Hou Jing-Yu, Yang Ping, Li Guang-Peng
机构信息
Jin-he Shi, Department of Emergency, The First Affiliated Hospital of Xinxiang Medical University, Weihui, China.
Li-dan Niu, Department of Emergency, The First Affiliated Hospital of Xinxiang Medical University, Weihui, China.
出版信息
Pak J Med Sci. 2015 Jan-Feb;31(1):198-202. doi: 10.12669/pjms.311.5997.
OBJECTIVE
We conducted a case-control study with 322 cases and 322 controls to assess the role of the two common SNPs in the promoter of IL-18 gene.
METHODS
Polymerase chain reaction restriction fragment length of polymorphism (PCR-RFLP) was taken to genotype -607A/C and -137C/G in the promoter of the IL-18 gene.
RESULTS
By comparing cases and control subjects, we found that IS cases were more likely to have higher BMI, higher proportion of hypertension, and have higher proportion of smokers and drinkers. We found that IL-18 -607CC genotype (OR=1.70, 95% CI=1.03-2.81) and C allele (OR=1.26, 95% CI=1.01-1.58) were significantly more frequent in IS patients when compared with AA genotype. We did not find significant association between IL-18 -607A/C gene polymorphism and BMI, hypertension, smoking and drinking on the risk of IS.
CONCLUSION
Our study suggests that polymorphisms in IL-18 -607A/C can influence the development of IS, and this gene polymorphism is associated with risk of IS in a Chinese population.
目的
我们进行了一项病例对照研究，纳入322例病例和322例对照，以评估白细胞介素-18（IL-18）基因启动子区域两个常见单核苷酸多态性（SNP）的作用。
方法
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对IL-18基因启动子区域的-607A/C和-137C/G进行基因分型。
结果
通过比较病例组和对照组，我们发现缺血性卒中（IS）病例更易出现较高的体重指数（BMI）、较高比例的高血压患者，以及较高比例的吸烟者和饮酒者。我们发现，与AA基因型相比，IL-18基因-607CC基因型（比值比[OR]=1.70，95%置信区间[CI]=1.03-2.81）和C等位基因（OR=1.26，95%CI=1.01-1.58）在IS患者中显著更常见。我们未发现IL-18基因-607A/C多态性与BMI、高血压、吸烟和饮酒对IS风险的影响之间存在显著关联。
结论
我们的研究表明，IL-18基因-607A/C多态性可影响IS的发生发展，且该基因多态性与中国人群的IS风险相关。
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