Yancheng Health Vocational and Technical College, Department of Medical Technology, Yancheng City, Jiangsu Province, China.
Braz J Med Biol Res. 2013 Jun;46(6):502-6. doi: 10.1590/1414-431X20132850. Epub 2013 May 24.
Single nucleotide polymorphisms in the promoter region of interleukin-18 (IL-18), an inflammatory cytokine, have been linked to susceptibility to many diseases, including cancer and immune dysfunction. Here, we explored the potential association between the IL-18 -607C/A (rs1946518) promoter region polymorphism and susceptibility to ischemic stroke (IS). This locus was amplified from peripheral blood samples of 386 IS patients (cases) and 364 healthy individuals (controls) by the polymerase chain reaction with sequence-specific primers. Significant differences were observed by the χ2 test in the -607C/A (rs1946518) genotype and allele frequencies between cases and controls (P < 0.05). Furthermore, after excluding for age, gender, smoking status, and hypertension, logistic regression indicated that IS susceptibility of -607C carriers increased 1.6 times (OR = 1.601, 95%CI = 1.148-2.233, P = 0.006) compared to -607A carriers. Additionally, similar increases in IS risk were noted for male patients or patients less than 65 years old. In conclusion, IL-18 -607C/A (rs1946518) promoter polymorphism is associated with IS susceptibility, and the C allele may confer increased IS risk.
白细胞介素-18（IL-18）启动子区域的单核苷酸多态性与许多疾病的易感性有关，包括癌症和免疫功能障碍。在这里，我们探讨了白细胞介素-18 -607C/A（rs1946518）启动子区域多态性与缺血性中风（IS）易感性之间的潜在关联。通过聚合酶链反应和序列特异性引物，从 386 名 IS 患者（病例）和 364 名健康个体（对照）的外周血样本中扩增了该基因座。卡方检验显示病例和对照组之间 -607C/A（rs1946518）基因型和等位基因频率存在显著差异（P<0.05）。此外，在排除年龄、性别、吸烟状况和高血压后，逻辑回归表明 -607C 携带者的 IS 易感性增加了 1.6 倍（OR=1.601，95%CI=1.148-2.233，P=0.006），而 -607A 携带者的 IS 易感性则无显著变化。此外，对于男性患者或年龄小于 65 岁的患者，IS 风险也有类似的增加。总之，白细胞介素-18 -607C/A（rs1946518）启动子多态性与 IS 易感性相关，C 等位基因可能增加 IS 风险。
