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红景天苷脂质体的制备条件优化及其对小鼠猪圆环病毒2型的免疫活性

Optimization on Preparation Conditions of Salidroside Liposome and Its Immunological Activity on PCV-2 in Mice.

作者信息

Feng Yibo, Zhao Xiaojuan, Lv Fang, Zhang Jinqiu, Deng Bihua, Zhao Yanhong, Hu Yuanliang, Wang Deyun, Liu Jiaguo, Lu Yu, Bo Ruonan, Liu Zhenguang

机构信息

Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.

National Research Center of Veterinary Biologicals Engineering and Technology, Jiangsu Academy of Agricultural Science, Nanjing 210014, China.

出版信息

Evid Based Complement Alternat Med. 2015;2015:178128. doi: 10.1155/2015/178128. Epub 2015 Mar 23.

DOI:10.1155/2015/178128
PMID:25878712
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4386707/
Abstract

The aim of this study was to optimize the preparation conditions of salidroside liposome with high encapsulation efficiency (EE) and to study the immunological enhancement activity of salidroside liposome as porcine circovirus type 2 virus (PCV-2) vaccine adjuvant. Response surface methodology (RSM) was selected to optimize the conditions for the preparation of salidroside liposome using Design-Expert V8.0.6 software. Three kinds of salidroside liposome adjuvants were prepared to study their adjuvant activity. BALB/c mice were immunized with PCV-2 encapsulated in different kinds of salidroside liposome adjuvants. The PCV-2-specific IgG in immunized mice serum was determined with ELISA. The results showed that when the concentration of ammonium sulfate was 0.26 mol·L(-1), ethanol volume 6.5 mL, temperature 43°C, ethanol injection rate 3 mL·min(-1), and salidroside liposome could be prepared with high encapsulation efficiency of 94.527%. Salidroside liposome as adjuvant could rapidly induce the production of PCV-2-specific IgG and salidroside liposome I adjuvant proved to provide the best effect among the three kinds of salidroside liposome adjuvants.

摘要

本研究旨在优化具有高包封率（EE）的红景天苷脂质体的制备条件，并研究红景天苷脂质体作为猪圆环病毒2型（PCV - 2）疫苗佐剂的免疫增强活性。采用响应面法（RSM），使用Design - Expert V8.0.6软件优化红景天苷脂质体的制备条件。制备了三种红景天苷脂质体佐剂并研究其佐剂活性。用包封于不同种类红景天苷脂质体佐剂中的PCV - 2免疫BALB/c小鼠。用ELISA法测定免疫小鼠血清中PCV - 2特异性IgG。结果表明，当硫酸铵浓度为0.26 mol·L⁻¹、乙醇体积为6.5 mL、温度为43℃、乙醇注入速率为3 mL·min⁻¹时，可制备出包封率高达94.527%的红景天苷脂质体。红景天苷脂质体作为佐剂可快速诱导PCV - 2特异性IgG的产生，并且红景天苷脂质体I佐剂在三种红景天苷脂质体佐剂中效果最佳。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/4386707/306df56d3061/ECAM2015-178128.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/4386707/637b964ad009/ECAM2015-178128.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/4386707/5fb84cc73847/ECAM2015-178128.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/4386707/1b1a587be2a8/ECAM2015-178128.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/4386707/306df56d3061/ECAM2015-178128.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/4386707/637b964ad009/ECAM2015-178128.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/4386707/5fb84cc73847/ECAM2015-178128.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/4386707/1b1a587be2a8/ECAM2015-178128.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/4386707/306df56d3061/ECAM2015-178128.004.jpg

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