Zhang Bei, Li Qiongqiong, Chu Xingkun, Sun Suya, Chen Shengdi
Department of Neurology and Institute of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin Er Road, Shanghai, 200025 China.
Laboratory of Neurodegenerative Diseases, Institute of Health Sciences, Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences (CAS) & Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai, 200025 China.
Transl Neurodegener. 2016 Nov 29;5:21. doi: 10.1186/s40035-016-0068-y. eCollection 2016.
Alzheimer's disease (AD) is an age-related and progressive neurodegenerative disease that causes substantial public health care burdens. Intensive efforts have been made to find effective and safe treatment against AD. Salidroside (Sal) is the main effective component of , which has several pharmacological activities. The objective of this study was to investigate the efficacy of Sal in the treatment of AD transgenic and the associated mechanisms.
We used tau transgenic line (TAU) in which tau protein is expressed in the central nervous system and eyes by the Gal4/UAS system. After feeding flies with Sal, the lifespan and locomotor activity were recorded. We further examined the appearance of vacuoles in the mushroom body using immunohistochemistry, and detected the levels of total glycogen synthase kinase 3β (t-GSK-3β), phosphorylated GSK-3β (p-GSK-3β), t-tau and p-tau in the brain by western blot analysis.
Our results showed that the longevity was improved in salidroside-fed groups as well as the locomotor activity. We also observed less vacuoles in the mushroom body, upregulated level of p-GSK-3β and downregulated p-tau following Sal treatment.
Our data presented the evidence that Sal was capable of reducing the neurodegeneration in tau transgenic and inhibiting neuronal loss. The neuroprotective effects of Sal were associated with its up-regulation of the p-GSK-3β and down-regulation of the p-tau.
阿尔茨海默病（AD）是一种与年龄相关的进行性神经退行性疾病，给公共卫生保健带来了沉重负担。人们一直在努力寻找有效且安全的AD治疗方法。红景天苷（Sal）是[具体药物名称]的主要有效成分，具有多种药理活性。本研究的目的是探讨红景天苷治疗AD转基因果蝇的疗效及其相关机制。
我们使用tau转基因果蝇品系（TAU），其中tau蛋白通过Gal4/UAS系统在中枢神经系统和眼睛中表达。用红景天苷喂养果蝇后，记录其寿命和运动活性。我们进一步通过免疫组织化学检查蘑菇体中液泡的出现情况，并通过蛋白质免疫印迹分析检测大脑中总糖原合酶激酶3β（t-GSK-3β）、磷酸化GSK-3β（p-GSK-3β）、总tau蛋白（t-tau）和磷酸化tau蛋白（p-tau）的水平。
我们的结果表明，喂食红景天苷的果蝇组寿命延长，运动活性也得到改善。我们还观察到，红景天苷处理后蘑菇体中的液泡减少，p-GSK-3β水平上调，p-tau下调。
我们的数据表明，红景天苷能够减少tau转基因果蝇的神经退行性变并抑制神经元丢失。红景天苷的神经保护作用与其上调p-GSK-3β和下调p-tau有关。
