Queiroga Marcos Roberto, Barbieri Ricardo Augusto, Ferreira Sandra Aires, Luchessi André Ducati, Hirata Rosario Dominguez Crespo, Hirata Mario Hiroyuki, Kokubun Eduardo
Departamento de Educação Física da Universidade Estadual do Centro-Oeste (UNICENTRO), Rua Simeão Camargo Varela de Sá, 03, 85040-080 Guarapuava, PR, Brazil.
Departamento de Educação Física da Universidade Estadual Paulista (UNESP), Avenida 24 A 1515, 13506-900 Rio Claro, SP, Brazil.
J Diabetes Res. 2015;2015:538732. doi: 10.1155/2015/538732. Epub 2015 Mar 24.
The influence of cardiorespiratory fitness (VO2max) on anthropometric variables and PPARG mRNA expression was investigated. Monozygotic twin pairs aged 11-18 years were grouped into discordant (D) and concordant (C) high and low VO2max groups. VO2max was determined by progressive maximal exercise test on treadmill with gas exchange analysis. Body mass (BM), BMI, waist circumference (WC), triceps (TR), and subscapular (SB) skinfold thicknesses were measured. Twins from the discordant group had differences in VO2max values (D-high = 45.9 ± 10.0 versus D-low = 32.4 ± 10.6 mL·kg(-1)·min(-1), P = 0.025), while no differences were found in the concordant group (C-high = 42.4 ± 9.2 versus C-low = 38.8 ± 9.8 mL·kg(-1)·min(-1), P = 0.952). In discordant group, VO2max was negatively correlated with TR + SB (r = -0.540, P = 0.021) and positively correlated with PPARG expression in leukocytes (r = 0.952, P = 0.001). Moreover, PPARG expression was directly correlated with BM (r = 0.714, P = 0.047) and height (r = 0.762, P = 0.028). In concordant twins, VO2max was inversely correlated with BM (r = -0.290, P = 0.027), BMI (r = -0.472, P = 0.001), WC (r = -0.426, P = 0.001), and TR + SB (r = -0.739, P = 0.001). Twins D-high had 1.78-fold greater PPARG expression when compared with twins D-low (P = 0.048). In conclusion, the cardiorespiratory fitness may modulate PPARG expression in childhood and adolescence, independently of the genetic background.
研究了心肺适能（最大摄氧量，VO2max）对人体测量学变量和PPARG mRNA表达的影响。将11至18岁的同卵双胞胎分为最大摄氧量高低不一致（D）组和一致（C）组。通过在跑步机上进行递增式最大运动测试并进行气体交换分析来测定最大摄氧量。测量体重（BM）、体重指数（BMI）、腰围（WC）、肱三头肌（TR）和肩胛下（SB）皮褶厚度。不一致组的双胞胎在最大摄氧量值上存在差异（D-高 = 45.9 ± 10.0 对比 D-低 = 32.4 ± 10.6 mL·kg(-1)·min(-1)，P = 0.025），而一致组未发现差异（C-高 = 42.4 ± 9.2 对比 C-低 = 38.8 ± 9.8 mL·kg(-1)·min(-1)，P = 0.952）。在不一致组中，最大摄氧量与TR + SB呈负相关（r = -0.540，P = 0.021），与白细胞中PPARG表达呈正相关（r = 0.952，P = 0.001）。此外，PPARG表达与BM（r = 0.714，P = 0.047）和身高（r = 0.762，P = 0.028）直接相关。在一致的双胞胎中，最大摄氧量与BM（r = -0.290，P = 0.027）、BMI（r = -0.472，P = 0.001）、WC（r = -0.426，P = 0.001）和TR + SB（r = -0.739，P = 0.001）呈负相关。与D-低的双胞胎相比，D-高的双胞胎PPARG表达高1.78倍（P = 0.048）。总之，心肺适能可能在儿童期和青少年期调节PPARG表达，独立于遗传背景。
