Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
Bioresour Technol. 2015;189:113-120. doi: 10.1016/j.biortech.2015.04.015. Epub 2015 Apr 8.
Organic loading rate (OLR) disturbances were introduced into a mesophilic anaerobic digester treating food waste (FW) to induce stable and deteriorative phases. The microbial community of each phase was investigated using 454-pyrosequencing. Results show that the relative abundance of acid-producing bacteria and syntrophic volatile fatty acid (VFA) oxidizers increased dramatically at deteriorative phase, while the dominant methanogens did not shift from acetoclastic to hydrogenotrophic groups. The mismatching between bacteria and methanogens may partially be responsible for the process deterioration. Moreover, the succession of predominant hydrogenotrophic methanogens reduced the consumption efficiency of hydrogen; meanwhile, the dominant Methanosaeta with low acetate degradation rate, and the increase of inhibitors concentrations further decreased its activity, which may be the other causes for the process failure. These results improve the understanding of the microbial mechanisms of process instability, and provide theoretical basis for the efficient and stable operation of anaerobic digester treating FW.
有机负荷率 (OLR) 干扰被引入到处理食物垃圾 (FW) 的中温厌氧消化器中，以诱导稳定和恶化阶段。使用 454 焦磷酸测序法研究了每个阶段的微生物群落。结果表明，在恶化阶段，产酸菌和共营养挥发性脂肪酸 (VFA) 氧化菌的相对丰度急剧增加，而主要的产甲烷菌并没有从乙酸营养型转变为氢营养型。细菌和产甲烷菌之间的不匹配可能是导致过程恶化的部分原因。此外，主要的氢营养型产甲烷菌的演替降低了氢气的消耗效率；同时，具有低乙酸降解率的优势 Methanosaeta 以及抑制剂浓度的增加进一步降低了其活性，这可能是过程失败的另一个原因。这些结果提高了对不稳定过程微生物机制的理解，并为高效稳定运行处理 FW 的厌氧消化器提供了理论依据。
