Weng Xuan, Zhao Wenting, Neethirajan Suresh, Duffield Todd
BioNano Laboratory, School of Engineering, University of Guelph, Guelph, ON, N1G 2W1, Canada.
Department of Industrial Engineering, South China University of Technology, Guangzhou, Guangdong, 510640, China.
J Nanobiotechnology. 2015 Feb 12;13:13. doi: 10.1186/s12951-015-0076-6.
Determination of β-hydroxybutyrate (βHBA) is a gold standard for diagnosis of Subclinical Ketosis (SCK), a common disease in dairy cows that causes significant economic loss. Early detection of SCK can help reduce the risk of the disease progressing into clinical stage, thus minimizing economic losses on dairy cattle. Conventional laboratory methods are time consuming and labor-intensive, requiring expensive and bulky equipment. Development of portable and robust devices for rapid on-site SCK diagnosis is an effective way to prevent and control ketosis and can significantly aid in the management of dairy animal health. Microfluidic technology provides a rapid, cost-effective way to develop handheld devices for on-farm detection of sub-clinical ketosis. In this study, a highly sensitive microfluidics-based biosensor for on-site SCK diagnosis has been developed.
A rapid, low-cost microfluidic biosensor with high sensitivity and specificity was developed for SCK diagnosis. Determination of βHBA was employed as the indicator in the diagnosis of SCK. On-chip detection using miniaturized and cost-effective optical sensor can be finished in 1 minute with a detection limit of 0.05 mM concentration. Developed microfluidic biosensor was successfully tested with the serum samples from dairy cows affected by SCK. The results of the developed biosensor agreed well with two other laboratory methods. The biosensor was characterized by high sensitivity and specificity towards βHBA with a detection limit of 0.05 mM.
The developed microfluidic biosensor provides a promising prototype for a cost-effective handheld meter for on-site SCK diagnosis. By using microfluidic method, the detection time is significantly decreased compared to other laboratory methods. Here, we demonstrate a field-deployable device to precisely identify and measure subclinical ketosis by specific labeling and quantification of β-hydroxybutyate in cow blood samples. A real-time on-site detection system will maximize convenience for the farmers.
β-羟基丁酸（βHBA）的测定是诊断亚临床酮病（SCK）的金标准，亚临床酮病是奶牛常见疾病，会造成重大经济损失。早期检测SCK有助于降低疾病发展到临床阶段的风险，从而将奶牛的经济损失降至最低。传统实验室方法耗时且劳动强度大，需要昂贵且体积庞大的设备。开发用于快速现场诊断SCK的便携式坚固设备是预防和控制酮病的有效方法，并且可以显著有助于奶牛健康管理。微流控技术为开发用于农场检测亚临床酮病的手持设备提供了一种快速、经济高效的方法。在本研究中，已开发出一种用于现场诊断SCK的高灵敏度基于微流控的生物传感器。
开发了一种用于SCK诊断的快速、低成本、具有高灵敏度和特异性的微流控生物传感器。将βHBA的测定用作SCK诊断的指标。使用小型化且经济高效的光学传感器进行芯片上检测可在1分钟内完成，检测限为0.05 mM浓度。所开发的微流控生物传感器已成功用受SCK影响的奶牛血清样本进行测试。所开发生物传感器的结果与其他两种实验室方法吻合良好。该生物传感器对βHBA具有高灵敏度和特异性，检测限为0.05 mM。
所开发的微流控生物传感器为用于现场SCK诊断的经济高效手持测量仪提供了一个有前景的原型。通过使用微流控方法，与其他实验室方法相比，检测时间显著减少。在此，我们展示了一种可现场部署的设备，通过对奶牛血样中的β-羟基丁酸进行特异性标记和定量来精确识别和测量亚临床酮病。实时现场检测系统将为农民带来最大便利。
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