钠离子和锂离子在大肠杆菌蜜二糖转运系统介导的硫代甲基半乳糖苷转运中的作用
Role of Na+ and Li+ in thiomethylgalactoside transport by the melibiose transport system of Escherichia coli.
作者信息
Lopilato J, Tsuchiya T, Wilson T H
出版信息
J Bacteriol. 1978 Apr;134(1):147-56. doi: 10.1128/jb.134.1.147-156.1978.
Abstract
Thiomethyl-beta-galactoside (TMG) accumulation via the melibiose transport system was studied in lactose transport-negative strains of Escherichia coli. TMG uptake by either intact cells or membrane vesicles was markedly stimulated by Na+ or Li+ between pH 5.5 and 8. The Km for uptake of TMG was approximately 0.2 mM at an external Na+ concentration of 5 mM (pH 7). The alpha-galactosides, melibiose, methyl-alpha-galactoside, and o-nitrophenyl-alpha-galactoside had a high affinity for this system whereas lactose, maltose and glucose had none. Evidence is presented for Li+-TMG or Na+-TMG cotransport.
摘要
通过蜜二糖转运系统对硫代甲基-β-半乳糖苷（TMG）积累进行了研究，研究对象为大肠杆菌乳糖转运阴性菌株。完整细胞或膜囊泡对TMG的摄取在pH 5.5至8之间受到Na⁺或Li⁺的显著刺激。在外部Na⁺浓度为5 mM（pH 7）时，TMG摄取的Km约为0.2 mM。α-半乳糖苷、蜜二糖、甲基-α-半乳糖苷和邻硝基苯基-α-半乳糖苷对该系统具有高亲和力，而乳糖、麦芽糖和葡萄糖则无此亲和力。有证据表明存在Li⁺-TMG或Na⁺-TMG共转运。
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Role of Na+ and Li+ in thiomethylgalactoside transport by the melibiose transport system of Escherichia coli.钠离子和锂离子在大肠杆菌蜜二糖转运系统介导的硫代甲基半乳糖苷转运中的作用
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