Wang Jianzhong, Cong Yanlong, Yin Renfu, Feng Na, Yang Songtao, Xia Xianzhu, Xiao Yueqiang, Wang Wenxiu, Liu Xiufan, Hu Shunlin, Ding Chan, Yu Shengqing, Wang Chunfeng, Ding Zhuang
Laboratory of Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun 130062, China; Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China.
Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun 130122, China.
Virus Res. 2015 May 4;203:77-83. doi: 10.1016/j.virusres.2015.04.006. Epub 2015 Apr 13.
Newcastle disease virus (NDV) and Goose parvovirus (GPV) are considered to be two of the most important and widespread viruses infecting geese. In this study, we generated a recombinant rmNA-VP3, expressing GPV VP3 using a modified goose-origin NDV NA-1 by changing the multi-basic cleavage site motif RRQKR↓F of the F protein to the dibasic motif GRQGR↓L as that of the avirulent strain LaSota as a vaccine vector. Expression of the VP3 protein in rmNA-VP3 infected cells was detected by immunofluorescence and Western blot assay. The genetic stability was examined by serially passaging 10 times in 10-day-old embryonated SPF chicken eggs. Goslings were inoculated with rmNA-VP3 showed no apparent signs of disease and developed a strong GPV and NDV neutralizing antibodies response. This is the first study demonstrating that recombinant NDV has the potential to serve as bivalent live vaccine against Goose parvovirus and Newcastle disease virus infection in birds.
新城疫病毒(NDV)和鹅细小病毒(GPV)被认为是感染鹅的两种最重要且分布广泛的病毒。在本研究中,我们构建了重组rmNA-VP3,通过将F蛋白的多碱性切割位点基序RRQKR↓F改变为无毒株LaSota的双碱性基序GRQGR↓L,利用改良的鹅源新城疫病毒NA-1作为疫苗载体表达GPV VP3。通过免疫荧光和蛋白质印迹分析检测rmNA-VP3感染细胞中VP3蛋白的表达。通过在10日龄SPF鸡胚中连续传代10次来检测遗传稳定性。接种rmNA-VP3的雏鹅未表现出明显的疾病迹象,并产生了强烈的GPV和NDV中和抗体反应。这是第一项证明重组新城疫病毒有潜力作为抗鹅细小病毒和新城疫病毒感染的鸟类双价活疫苗的研究。