Yuan Yi, Zhang Yaqin, Liu Bin, Wu Heming, Kang Yanjun, Li Ming, Zeng Xin, He Nongyue, Zhang Gen
Institute of Stomatology, Nanjing Medical University, Nanjing, 210029, China.
Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, 210029, China.
J Nanobiotechnology. 2015 Feb 12;13:12. doi: 10.1186/s12951-015-0070-z.
Nano drugs have attracted increased attention due to their unique mode of action that offers tumor-inhibiting effects. Therefore, we have previously explored functionalized and drug-loaded graphene-gold nanocomposites that induced cancer cell apoptosis.
In the present study, we developed a combination of monoclonal P-glycoprotein (P-gp) antibodies, folic acid (FA) and miR-122-loaded gold nanoparticles on graphene nanocomposites (GGMPN), which promoted drug-resistant HepG2 cell apoptosis with drug targeting and controlled release properties. We also investigated related apoptosis proteins and apoptosis signal pathways by GGMPN treatment in vitro and in vivo. Moreover, we further demonstrated the inhibition of tumor growth and the apoptosis-inducing effect by means of GGMPN with a semiconductor laser in a xenograft tumor model.
In conclusion, our results collectively suggested that GGMPN could serve as a novel therapeutic approach to control tumor cell apoptosis and growth.
纳米药物因其独特的作用方式而具有肿瘤抑制作用，从而受到越来越多的关注。因此，我们之前探索了功能化且负载药物的石墨烯-金纳米复合材料，其可诱导癌细胞凋亡。
在本研究中，我们在石墨烯纳米复合材料（GGMPN）上开发了单克隆P-糖蛋白（P-gp）抗体、叶酸（FA）和负载miR-122的金纳米颗粒的组合，其具有药物靶向和控释特性，可促进耐药性肝癌细胞凋亡。我们还通过体外和体内GGMPN处理研究了相关凋亡蛋白和凋亡信号通路。此外，我们在异种移植肿瘤模型中进一步证明了GGMPN联合半导体激光对肿瘤生长的抑制作用和诱导凋亡的效果。
总之，我们的结果共同表明，GGMPN可作为一种控制肿瘤细胞凋亡和生长的新型治疗方法。
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