γ-生育三烯酚处理可增加肝癌细胞系HepG2中过氧化物酶体增殖物激活受体4的表达。
Gamma-tocotrienol treatment increased peroxiredoxin-4 expression in HepG2 liver cancer cell line.
作者信息
Abdul Rahman Sazli Farahani, Jubri Zakiah, Abdul Rahman Mariati, Karsani Saiful Anuar, Md Top Abdul Gapor, Wan Ngah Wan Zurinah
机构信息
Department of Biochemistry, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia.
Department of Clinical Oral Biology, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
出版信息
BMC Complement Altern Med. 2015 Mar 13;15:64. doi: 10.1186/s12906-015-0590-y.
BACKGROUND
To determine the antiproliferative effect of gamma-tocotrienol (GTT) treatment on differential protein expression in HepG2 cells.
METHODS
HepG2 cells were treated with 70 μM GTT for 48 hours and differentially expressed protein spots were determined by two-dimensional electrophoresis (2DE), identified by MALDI-TOF mass spectrometer (MS) and validated by quantitative real-time polymerase chain reaction (qRT-PCR).
RESULTS
GTT treatment on HepG2 cells showed a total of five differentially expressed proteins when compared to their respective untreated cells where three proteins were down-regulated and two proteins were up-regulated. One of these upregulated proteins was identified as peroxiredoxin-4 (Prx4). Validation by qRT-PCR however showed decreased expression of Prx4 mRNA in HepG2 cells following GTT treatment.
CONCLUSIONS
GTT might directly influence the expression dynamics of peroxiredoxin-4 to control proliferation in liver cancer.
背景
确定γ-生育三烯酚（GTT）处理对HepG2细胞中差异蛋白表达的抗增殖作用。
方法
用70μM GTT处理HepG2细胞48小时，通过二维电泳（2DE）确定差异表达的蛋白点，用基质辅助激光解吸电离飞行时间质谱仪（MALDI-TOF MS）进行鉴定，并通过定量实时聚合酶链反应（qRT-PCR）进行验证。
结果
与各自未处理的细胞相比，GTT处理HepG2细胞显示共有5种差异表达蛋白，其中3种蛋白下调，2种蛋白上调。这些上调蛋白之一被鉴定为过氧化物酶体增殖物激活受体4（Prx4）。然而，qRT-PCR验证显示，GTT处理后HepG2细胞中Prx4 mRNA的表达降低。
结论
GTT可能直接影响过氧化物酶体增殖物激活受体4的表达动态，以控制肝癌细胞的增殖。
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