Ude C C, Ng M H, Chen C H, Htwe O, Amaramalar N S, Hassan S, Djordjevic I, Rani R A, Ahmad J, Yahya N M, Saim A B, Idrus R B Hj
Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, 56000 K.L, Malaysia; Department of Physiology, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, Cheras 56000, K. L, Malaysia.
Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, 56000 K.L, Malaysia.
Osteoarthritis Cartilage. 2015 Aug;23(8):1294-306. doi: 10.1016/j.joca.2015.04.003. Epub 2015 Apr 14.
Our previous studies on osteoarthritis (OA) revealed positive outcome after chondrogenically induced cells treatment. Presently, the functional improvements of these treated OA knee joints were quantified followed by evaluation of the mechanical properties of the engineered cartilages.
Baseline electromyogram (EMGs) were conducted at week 0 (pre-OA), on the locomotory muscles of nine un-castrated male sheep (Siamese long tail cross) divided into controls, adipose-derived stem cells (ADSCs) and bone marrow stem cells (BMSCs), before OA inductions. Subsequent recordings were performed at week 7 and week 31 which were post-OA and post-treatments. Afterwards, the compression tests of the regenerated cartilage were performed.
Post-treatment EMG analysis revealed that the control sheep retained significant reductions in amplitudes at the right medial gluteus, vastus lateralis and bicep femoris, whereas BMSCs and ADSCs samples had no further significant reductions (P < 0.05). Grossly and histologically, the treated knee joints demonstrated the presence of regenerated neo cartilages evidenced by the fluorescence of PKH26 tracker. Based on the International Cartilage Repair Society scores (ICRS), they had significantly lower grades than the controls (P < 0.05). The compression moduli of the native cartilages and the engineered cartilages differed significantly at the tibia plateau, patella femoral groove and the patella; whereas at the medial femoral condyle, they had similar moduli of 0.69 MPa and 0.40-0.64 MPa respectively. Their compression strengths at all four regions were within ±10 MPa.
The tissue engineered cartilages provided evidence of functional recoveries associated to the structural regenerations, and their mechanical properties were comparable with the native cartilage.
我们之前对骨关节炎（OA）的研究表明，经软骨诱导细胞治疗后有积极的结果。目前，对这些接受治疗的OA膝关节的功能改善进行了量化，随后评估了工程化软骨的力学性能。
在第0周（OA诱导前），对9只未阉割的雄性绵羊（暹罗长尾杂交）的运动肌肉进行基线肌电图（EMG）检查，这些绵羊被分为对照组、脂肪来源干细胞（ADSCs）组和骨髓干细胞（BMSCs）组。随后在第7周和第31周进行记录，分别是OA后和治疗后。之后，对再生软骨进行压缩试验。
治疗后EMG分析显示，对照绵羊的右臀中肌、股外侧肌和股二头肌的振幅仍显著降低，而BMSCs和ADSCs样本的振幅没有进一步显著降低（P<0.05）。大体和组织学上，治疗后的膝关节显示存在再生的新软骨，PKH26追踪剂的荧光证明了这一点。根据国际软骨修复协会评分（ICRS），它们的等级明显低于对照组（P<0.05）。天然软骨和工程化软骨的压缩模量在胫骨平台、髌股沟和髌骨处有显著差异；而在内侧股骨髁处，它们的模量相似，分别为0.69MPa和0.40 - 0.64MPa。它们在所有四个区域的压缩强度都在±10MPa以内。
组织工程化软骨为与结构再生相关的功能恢复提供了证据，并且它们的力学性能与天然软骨相当。
