Yun Sungho, Ku Sae-Kwang, Kwon Young-Sam
Department of Veterinary Surgery, College of Veterinary Medicine, Kyungpook National University, Daegu, 702-701, Republic of Korea.
Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University, Gyeongsan, 712-715, Republic of Korea.
J Orthop Surg Res. 2016 Jan 15;11:9. doi: 10.1186/s13018-016-0342-9.
The purpose of this study is to investigate the clinical effects of platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cell (MSC) as the fundamental treatment of osteoarthritis (OA).
Twenty four Beagle dogs were used as cranial cruciate ligament transection models. The dogs were divided into four groups (n = 6) according to the intra-articular injection materials: the control group with phosphate-buffered saline (PBS), the PRP group with PRP, the MSC group with MSCs emerged in PBS, and the MSC and PRP co-treatment (MP) group with MSCs and PRP.
Lameness score, focal compression strength, articular extracellular matrix (ECM) compositions, histopathology, and real-time PCR were used to evaluate the effects of PRP and MSCs on canine OA. In the order of MP, PRP, and MSC group, these all showed positive effects on the evaluated categories. The lameness scores were lower, and the focal compression strengths of the affected femoral articular surface cartilages were higher than those in the OA control group. Also, the inflammatory changes, when evaluated with Mankin scoring and histomorphologic examination, were significantly ameliorated with the treatment of PRP and/or MSCs. The glycosaminoglycan and collagen composition of extracellular matrix was more favorable in the test groups. The ECM-related genes significantly increased through the up-regulation, while the protein expressions of inflammatory cytokines were decreased through the inhibitory effects of PRP and MSCs on chondrocyte apoptosis and inflammatory cytokines.
Taken together, this study suggests that PRP and MSCs treatments have a beneficial effect on OA via the stimulation of ECM synthesis and chondrocyte proliferation and via the inhibition of inflammatory reaction.
本研究旨在探讨富血小板血浆（PRP）和脂肪间充质干细胞（MSC）作为骨关节炎（OA）基础治疗的临床效果。
将24只比格犬作为前交叉韧带切断模型。根据关节内注射材料将犬分为四组（n = 6）：用磷酸盐缓冲盐水（PBS）的对照组、用PRP的PRP组、用PBS中出现的MSC的MSC组以及用MSC和PRP的MSC与PRP联合治疗（MP）组。
采用跛行评分、局部抗压强度、关节细胞外基质（ECM）成分、组织病理学和实时PCR来评估PRP和MSC对犬OA的影响。按MP、PRP和MSC组的顺序，这些在评估类别上均显示出积极效果。跛行评分较低，患侧股骨关节面软骨的局部抗压强度高于OA对照组。此外，用曼金评分和组织形态学检查评估时，PRP和/或MSC治疗可显著改善炎症变化。测试组细胞外基质的糖胺聚糖和胶原蛋白组成更有利。ECM相关基因通过上调显著增加，而炎症细胞因子的蛋白表达通过PRP和MSC对软骨细胞凋亡和炎症细胞因子的抑制作用而降低。
综上所述，本研究表明PRP和MSC治疗通过刺激ECM合成和软骨细胞增殖以及抑制炎症反应对OA具有有益作用。
