Zhang Jinpeng, Liu Weihua, Han Haiming, Song Liqiang, Bai Li, Gao Zhihui, Zhang Yan, Yang Xinming, Li Xiuquan, Gao Ainong, Li Lihui
National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Genomics. 2015 Aug;106(2):129-36. doi: 10.1016/j.ygeno.2015.04.003. Epub 2015 Apr 15.
Agropyron cristatum is a wild grass of the tribe Triticeae that is widely grown in harsh environments. As a wild relative of wheat, A. cristatum carries many resistance genes that could be used to broaden the genetic diversity of wheat. Here, we report the transcriptome sequencing of the flag leaf and young spike tissues of a representative tetraploid A. cristatum. More than 90 million reads from the two tissues were assembled into 73,664 unigenes. All unigenes were functionally annotated against the KEGG, COG, and Gene Ontology databases and predicted long non-coding RNAs. Pfam prediction demonstrates that A. cristatum carries an abundance of stress resistance genes. The extent of specific genes and rare alleles make A. cristatum a vital genetic reservoir for the improvement of wheat. Altogether, the available gene resources in A. cristatum facilitate efforts to harness the genetic diversity of wild relatives to enhance wheat.
冰草是小麦族的一种野草，广泛生长于恶劣环境中。作为小麦的野生近缘种，冰草携带许多抗性基因，可用于拓宽小麦的遗传多样性。在此，我们报告了一个代表性四倍体冰草旗叶和幼穗组织的转录组测序。来自这两个组织的9000多万条 reads 被组装成73,664个单基因。所有单基因都根据KEGG、COG和基因本体数据库进行了功能注释，并预测了长链非编码RNA。Pfam预测表明，冰草携带丰富的抗逆基因。特定基因和稀有等位基因的存在使冰草成为改良小麦的重要基因库。总之，冰草中可用的基因资源有助于利用野生近缘种的遗传多样性来改良小麦。
