Yadav Dhananjay, Mishra Meerambika, Joseph Anish Zacharia, Subramani Senthil Kumar, Mahajan Sunil, Singh Nita, Bisen Prakash Singh, Prasad G B K S
Department of Internal Medicine, Yonsei University Wonju College of Medicine, Wonju, South Korea; SOS in Biochemistry Jiwaji University, Gwalior, Madhya Pradesh, India.
School of Life Sciences, Sambalpur University, Jyoti Vihar, Burla, Odisha, India.
Obes Res Clin Pract. 2015 Mar-Apr;9(2):158-67. doi: 10.1016/j.orcp.2014.03.004. Epub 2014 May 1.
Antioxidants play a very crucial role in terms of disease incidence and its complications. Type 2 diabetes and metabolic syndrome (MetS) are now more prevalent than any other disorder. In this regard this study focuses on type 2 diabetic subjects with MetS having highest incidence of cardiovascular disease. Hence the aim of the study was to reveal the level of antioxidants and lipid peroxidation in MetS and non-MetS groups of type 2 diabetic subjects characterized by three different criteria.
Present study was based on 70 type 2 diabetic subjects and 20 healthy controls. The metabolic syndrome criteria were defined by National Cholesterol Education Program (NCEP)-Adult Treatment Panel III (ATPIII), International Diabetes Federation (IDF), and World Health Organization (WHO). The selected subjects were of similar age group. The participants were selected from a diabetic camp being run in the University Campus. The antioxidant enzymes estimated were reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) while Thiobarbituric acid reactive substances (TBARS) measured as a marker of lipid peroxidation.
The level of GSH, CAT, SOD decreases in non-MetS and MetS subjects as compared to normal. When comparison was made for GSH & SOD in the mentioned groups resulted a significant differences in non-MetS and MetS compared with normal subjects. TBARS levels was increased in non-MetS and MetS group of subjects. The results indicate reduction in antioxidant enzymes and elevation of lipid peroxidation in type 2 diabetic subjects with or without MetS defined by using any of the International criteria.
The study revealed that absolute reduction of antioxidant and involvement of lipid peroxidation in MetS group may lead to progressive intensification of cardiovascular disease in type 2 diabetic subjects irrespective of using any criteria.
抗氧化剂在疾病的发生及其并发症方面起着至关重要的作用。2型糖尿病和代谢综合征（MetS）现在比任何其他疾病都更普遍。在这方面，本研究聚焦于患有心血管疾病发病率最高的2型糖尿病合并代谢综合征的受试者。因此，本研究的目的是揭示以三种不同标准为特征的2型糖尿病受试者的代谢综合征组和非代谢综合征组中的抗氧化剂水平和脂质过氧化情况。
本研究基于70名2型糖尿病受试者和20名健康对照者。代谢综合征标准由美国国家胆固醇教育计划（NCEP）成人治疗小组第三次报告（ATPIII）、国际糖尿病联盟（IDF）和世界卫生组织（WHO）定义。所选受试者为相似年龄组。参与者从大学校园举办的糖尿病营地中选取。所评估的抗氧化酶为还原型谷胱甘肽（GSH）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD），而硫代巴比妥酸反应性物质（TBARS）作为脂质过氧化的标志物进行测量。
与正常组相比，非代谢综合征组和代谢综合征组受试者的GSH、CAT、SOD水平降低。在上述组中对GSH和SOD进行比较时，非代谢综合征组和代谢综合征组与正常受试者相比存在显著差异。非代谢综合征组和代谢综合征组受试者的TBARS水平升高。结果表明，无论使用任何国际标准定义，患有或未患有代谢综合征的2型糖尿病受试者的抗氧化酶均减少，脂质过氧化增加。
该研究表明，无论使用何种标准，代谢综合征组中抗氧化剂的绝对减少和脂质过氧化的参与可能导致2型糖尿病受试者心血管疾病的逐渐加重。
