Matkovics B, Kotormán M, Varga I S, Hai D Q, Salgó L, Novák Z
Biological Isotope Laboratory, József Attila University of Szeged, Hungary.
Acta Physiol Hung. 1997;85(2):107-12.
This study was conducted on type 2 non-insulin-dependent diabetes mellitus (NIDDM) cases and healthy blood donors. Lipid peroxidation (LP) products in plasma and red blood cell (RBC) hemolysates were estimated as total thiobarbituric acid reactive substances (TTBARS). The plasma and hemolysate reduced and oxidized glutathione (GSH and GSSG) levels are compared. In the hemolysates the antioxidant enzymes namely superoxide dismutase (SOD), glutathione peroxidase (GPx-ase), glutathione reductase (GR-ase) and catalase (C-ase) are also compared. The RBC filtration characteristics are determined and compared with controls: 1. LP and GSH in diabetic plasma were significantly higher, but in the hemolysate the GSH raised but the LP was significantly lower in diabetics than in healthy controls. 2. Superoxide dismutase and C-ase were significantly higher in NIDDM hemolysate. Contrary the GPx-ase activity was significantly lower in diabetics. 3. The diabetic RBCs filtration characteristics are changed in respects significantly namely the Fi was lower, the Tc and CR were higher. It means higher rigidity and oxidative damage of the membrane of diabetic RBCs.
本研究针对2型非胰岛素依赖型糖尿病（NIDDM）患者和健康献血者展开。血浆和红细胞（RBC）溶血产物中的脂质过氧化（LP）产物以总硫代巴比妥酸反应性物质（TTBARS）进行估算。比较了血浆和溶血产物中还原型和氧化型谷胱甘肽（GSH和GSSG）的水平。在溶血产物中，还比较了抗氧化酶，即超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx - ase）、谷胱甘肽还原酶（GR - ase）和过氧化氢酶（C - ase）。测定了红细胞的过滤特性并与对照组进行比较：1. 糖尿病患者血浆中的LP和GSH显著更高，但在溶血产物中，糖尿病患者的GSH升高，而LP显著低于健康对照组。2. NIDDM溶血产物中的超氧化物歧化酶和C - ase显著更高。相反，糖尿病患者的GPx - ase活性显著更低。3. 糖尿病患者红细胞的过滤特性在各方面均有显著变化，即滤过指数（Fi）更低，滤过时间（Tc）和滤过率（CR）更高。这意味着糖尿病患者红细胞膜的刚性更高且存在氧化损伤。
