Milton Ross D, Lim Koun, Hickey David P, Minteer Shelley D
Departments of Chemistry and Materials Science and Engineering, University of Utah, 315 S 1400 E Room 2020, Salt Lake City, UT 84112, United States.
Departments of Chemistry and Materials Science and Engineering, University of Utah, 315 S 1400 E Room 2020, Salt Lake City, UT 84112, United States.
Bioelectrochemistry. 2015 Dec;106(Pt A):56-63. doi: 10.1016/j.bioelechem.2015.04.005. Epub 2015 Apr 9.
Flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) is emerging as an oxygen-insensitive alternative to glucose oxidase (GOx) as the biocatalyst for bioelectrodes and bioanodes in glucose sensing and glucose enzymatic fuel cells (EFCs). Glucose EFCs, which utilize oxygen as the oxidant and final electron acceptor, have the added benefit of being able to be implanted within living hosts. These can then produce electrical energy from physiological glucose concentrations and power internal or external devices. EFCs were prepared with FAD-GDH and bilirubin oxidase (BOx) to evaluate the suitability of FAD-GDH within an implantable setting. Maximum current and power densities of 186.6±7.1 μA cm(-2) and 39.5±1.3 μW cm(-2) were observed when operating in human serum at 21 °C, which increased to 285.7±31.3 μA cm(-2) and 57.5±5.4 μW cm(-2) at 37 °C. Although good stability was observed with continual near-optimal operation of the EFCs in human serum at 21 °C for 24 h, device failure was observed between 13-14 h when continually operated at 37 °C.
黄素腺嘌呤二核苷酸依赖性葡萄糖脱氢酶（FAD-GDH）作为一种对氧气不敏感的酶，正逐渐成为葡萄糖氧化酶（GOx）的替代物，可作为葡萄糖传感和葡萄糖酶燃料电池（EFC）中生物电极和生物阳极的生物催化剂。利用氧气作为氧化剂和最终电子受体的葡萄糖EFC具有能够植入活体宿主的额外优势。这些电池随后可以从生理葡萄糖浓度产生电能，并为内部或外部设备供电。制备了含有FAD-GDH和胆红素氧化酶（BOx）的EFC，以评估FAD-GDH在可植入环境中的适用性。在21°C的人血清中运行时，观察到最大电流密度和功率密度分别为186.6±7.1μA cm(-2)和39.5±1.3μW cm(-2)，在37°C时分别增加到285.7±31.3μA cm(-2)和57.5±5.4μW cm(-2)。尽管在21°C的人血清中，EFC持续近最优运行24小时表现出良好的稳定性，但在37°C持续运行时，在13-14小时之间观察到设备故障。
