Perry Robert D, Bobrov Alexander G, Fetherston Jacqueline D
Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, KY, USA.
Metallomics. 2015 Jun;7(6):965-78. doi: 10.1039/c4mt00332b.
Yersinia pestis, the causative agent of bubonic, septicemic and pneumonic plague, encodes a multitude of Fe transport systems. Some of these are defective due to frameshift or IS element insertions, while others are functional in vitro but have no established role in causing infections. Indeed only 3 Fe transporters (Ybt, Yfe and Feo) have been shown to be important in at least one form of plague. The yersiniabactin (Ybt) system is essential in the early dermal/lymphatic stages of bubonic plague, irrelevant in the septicemic stage, and critical in pneumonic plague. Two Mn transporters have been characterized (Yfe and MntH). These two systems play a role in bubonic plague but the double yfe mntH mutant is fully virulent in a mouse model of pneumonic plague. The same in vivo phenotype occurs with a mutant lacking two (Yfe and Feo) of four ferrous transporters. A role for the Ybt siderophore in Zn acquisition has been revealed. Ybt-dependent Zn acquisition uses a transport system completely independent of the Fe-Ybt uptake system. Together Ybt components and ZnuABC play a critical role in Zn acquisition in vivo. Single mutants in either system retain high virulence in a mouse model of septicemic plague while the double mutant is completely avirulent.
鼠疫耶尔森菌是腺鼠疫、败血性鼠疫和肺鼠疫的病原体，它编码多种铁转运系统。其中一些由于移码突变或插入序列元件而存在缺陷，而其他一些在体外具有功能，但在引起感染方面尚未明确其作用。实际上，只有3种铁转运蛋白（yersiniabactin，Ybt；yersiniabactin铁转运蛋白，Yfe；铁转运蛋白O，Feo）已被证明在至少一种鼠疫形式中很重要。yersiniabactin（Ybt）系统在腺鼠疫的早期皮肤/淋巴阶段至关重要，在败血性鼠疫阶段无关紧要，而在肺鼠疫中则至关重要。已经鉴定出两种锰转运蛋白（Yfe和锰转运蛋白H，MntH）。这两个系统在腺鼠疫中发挥作用，但双基因yfe mntH突变体在肺鼠疫小鼠模型中具有完全的毒力。在缺乏四种亚铁转运蛋白中的两种（Yfe和Feo）的突变体中也出现了相同的体内表型。已经揭示了Ybt铁载体在锌摄取中的作用。依赖Ybt的锌摄取使用一个完全独立于铁-Ybt摄取系统的转运系统。Ybt组分和锌转运蛋白ABC（ZnuABC）共同在体内锌摄取中起关键作用。任一系统中的单突变体在败血性鼠疫小鼠模型中都保持高毒力，而双突变体则完全无毒。
