Fusco Salvatore, Liguori Rossana, Limauro Danila, Bartolucci Simonetta, She Qunxin, Contursi Patrizia
Dipartimento di Biologia, Università degli Studi di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cinthia, 80126 Napoli, Italy.
Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cinthia, 80126 Napoli, Italy.
Biochimie. 2015 Nov;118:322-32. doi: 10.1016/j.biochi.2015.04.006. Epub 2015 Apr 17.
Fuselloviruses SSV1 and SSV2 are model systems to investigate virus-host relationships in stably infected cells thanks to their temperate nature. Although they are very similar in morphology, genome organization and gene synteny, their replication is induced by different stimuli, i.e.: by UV-light exposure (for SSV1) and by the growth progression of the host (for SSV2). In this study, we have analysed global gene expression in SSV1- and SSV2-lysogens of Sulfolobus solfataricus P2 in the absence of any stimuli. Additionally, the interplay among SSV1, SSV2 and the host has been investigated in a double-infected strain to explore both virus-host and virus-virus interactions. Whereas SSV1 did not induce major changes of the host gene expression, SSV2 elicited a strong host response, which includes the transcriptional activation of CRISPR loci and cas genes. As a consequence, a significant decrease of the SSV2 copy number has been observed, which in turn led to provirus-capture into the host chromosome. Results of this study have revealed novel aspects of the host-viral interaction in the frame of the CRISPR-response.
由于温和噬菌体的特性，丝状病毒SSV1和SSV2是研究稳定感染细胞中病毒与宿主关系的模型系统。尽管它们在形态、基因组组织和基因共线性方面非常相似，但它们的复制由不同的刺激诱导，即：紫外线照射（对于SSV1）和宿主的生长进程（对于SSV2）。在本研究中，我们分析了在没有任何刺激的情况下，嗜热栖热菌P2的SSV1和SSV2溶原菌中的全局基因表达。此外，还在双重感染菌株中研究了SSV1、SSV2与宿主之间的相互作用以探索病毒-宿主和病毒-病毒相互作用。虽然SSV1没有引起宿主基因表达发生重大变化，但SSV2引发了强烈的宿主反应，其中包括CRISPR位点和cas基因的转录激活。结果，观察到SSV2拷贝数显著减少，这反过来导致前病毒捕获到宿主染色体中。本研究结果揭示了CRISPR反应框架内宿主-病毒相互作用的新方面。
