Fröls Sabrina, Gordon Paul M K, Panlilio Mayi Arcellana, Schleper Christa, Sensen Christoph W
University of Bergen, Department of Biology, Jahnebakken 5, N-5020 Bergen, Norway.
Virology. 2007 Aug 15;365(1):48-59. doi: 10.1016/j.virol.2007.03.033. Epub 2007 Apr 30.
The spindle-shaped Sulfolobus virus SSV1 was the first of a series of unusual and uniquely shaped viruses isolated from hyperthermophilic Archaea. Using whole-genome microarrays we show here that the circular 15.5 kb DNA genome of SSV1 exhibits a chronological regulation of its transcription upon UV irradiation, reminiscent to the life cycles of bacteriophages and eukaryotic viruses. The transcriptional cycle starts with a small UV-specific transcript and continues with early transcripts on both its flanks. The late transcripts appear after the onset of viral replication and are extended to their full lengths towards the end of the approximately 8.5 h cycle. While we detected only small differences in genome-wide analysis of the host Sulfolobus solfataricus comparing infected versus uninfected strains, we found a marked difference with respect to the strength and speed of the general UV response of the host. Models for the regulation of the virus cycle, and putative functions of genes in SSV1 are presented.
纺锤形的硫化叶菌病毒SSV1是从嗜热古菌中分离出的一系列形状异常且独特的病毒中的首个病毒。我们在此使用全基因组微阵列表明，SSV1的环状15.5 kb DNA基因组在紫外线照射后其转录呈现出时间顺序调控，这让人联想到噬菌体和真核病毒的生命周期。转录周期始于一个小的紫外线特异性转录本，接着是其两侧的早期转录本。晚期转录本在病毒复制开始后出现，并在大约8.5小时的周期结束时延伸至全长。虽然在对感染与未感染菌株的宿主嗜热栖热菌进行全基因组分析时我们仅检测到微小差异，但我们发现宿主的一般紫外线反应在强度和速度方面存在显著差异。本文还提出了病毒周期调控模型以及SSV1中基因的推定功能。
