†Hamburg School of Food Science; Institute of Food Chemistry, University of Hamburg, Grindelallee 117, 20146 Hamburg, Germany.
‡Biozentrum Klein Flottbek, University of Hamburg, Ohnhorststr. 18, 22609 Hamburg, Germany.
J Agric Food Chem. 2015 May 13;63(18):4539-44. doi: 10.1021/acs.jafc.5b01462. Epub 2015 May 1.
The cocoa type "Colección Castro Naranjal 51" (CCN-51) is known for its resistance to specific climate conditions and its high yield, but it shows a weaker flavor profile and therefore is marketed as bulk cocoa. In a previous study, the two cocoa types Arriba and CCN-51 could easily be distinguished, but differences among the CCN-51 samples were observed. This was unexpected, as CCN-51 is reported to be a clone. To confirm whether CCN-51 is a pure clone, 10 simple sequence repeats (SSR) located on the nuclear genome were used to analyze various CCN-51 samples in comparison to the cocoa varieties Arriba and Criollo. As expected, there are differences in the SSR pattern among CCN-51, Arriba, and Criollo, but a variability within the CCN-51 sample set was detected as well. The previously described sequence variation in the chloroplast genome was confirmed by a variability in the microsatellite loci of the nuclear genome for a comprehensive cultivar collection of CCN-51 of both bean and leaf samples. In summary, beneath somaclonal variation, misidentification of plant collections and also sexual reproduction of CCN-51 can be suggested.
可可品种“Castro Naranjal 51 收藏系列”（CCN-51）以其对特定气候条件的抗性和高产量而闻名，但它的风味特征较弱，因此作为散装可可销售。在之前的一项研究中，两种可可品种 Arriba 和 CCN-51 很容易区分，但观察到 CCN-51 样本之间存在差异。这令人意外，因为据报道 CCN-51 是一个克隆品种。为了确认 CCN-51 是否是一个纯克隆品种，使用了位于核基因组上的 10 个简单序列重复（SSR）来分析与可可品种 Arriba 和 Criollo 相比的各种 CCN-51 样本。正如预期的那样，CCN-51、Arriba 和 Criollo 之间的 SSR 模式存在差异，但也检测到 CCN-51 样本集中存在可变性。先前在叶绿体基因组中描述的序列变异通过核基因组微卫星标记的变异得到了证实，这些微卫星标记用于 CCN-51 的综合品种收集，包括豆样和叶片样本。总之，除了体细胞变异之外，还可以推测 CCN-51 的植物收集存在错误识别和有性繁殖。
