Wang Hongzhi, Wang Yu, Liu Su, Yu Jinghua, Xu Wei, Guo Yuna, Huang Jiadong
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China.
Chem Commun (Camb). 2015 May 14;51(39):8377-80. doi: 10.1039/c5cc01473e.
A novel electrochemical aptasensor for ultrasensitive detection of antibiotics by combining polymerase-assisted target recycling amplification with strand displacement amplification with the help of polymerase and nicking endonuclease has been reported. This work is the first time that target-aptamer binding triggered quadratic recycling amplification has been utilized for electrochemical detection of antibiotics.
据报道,一种新型电化学适体传感器,借助聚合酶和切口内切酶,通过将聚合酶辅助的靶标循环扩增与链置换扩增相结合,用于超灵敏检测抗生素。这项工作首次将靶标-适体结合触发的二次循环扩增用于抗生素的电化学检测。