Yin Jingfang, Yang Xi, Zeng Qi, Yang Linglan, Cheng Bin, Tao Xiaoan
Department of Oral Medicine, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, China.
Department of Periodontology, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, China.
J Oral Pathol Med. 2016 Jan;45(1):41-7. doi: 10.1111/jop.12325. Epub 2015 Apr 20.
The important roles of CCL2 and its receptor CCR2 had been reported in a series of inflammatory disorders. However, few studies investigated the potential role of CCL2/CCR2 axis in oral lichen planus (OLP). Therefore, this study aimed to detect the expression of CCL2 and CCR2 in OLP lesions and compare their changes before and after treatment.
CCL2 and CCR2 expression was investigated using immunohistochemical staining and real-time RT-PCR in 32 patients with OLP and eight controls. Moreover, changes in their expression after treatment with triamcinolone acetonide were assessed in lesions from three patients.
CCL2+ and CCR2+ cells were few in the controls and remarkably increased in the epithelial and subepithelial layers of lesions (n = 32, all P < 0.001). However, the densities of CCL2+ and CCR2+ cells were not significantly different between reticular (n = 12) and erythematous/erosive lesions (n = 20), although they significantly decreased after treatment (627.7 ± 108.2 vs. 258.3 ± 148.3, P = 0.017; 1034.7 ± 74.6 vs. 648 ± 77.6, P = 0.003, respectively). CCL2+/CCR2+ cell numbers were positively correlated with disease activity (correlation coefficient, 0.588; P < 0.001; correlation coefficient, 0.409; P = 0.02, respectively).
The results of this study indicated that the CCL2-CCR2 axis was involved in the pathogenesis of OLP and was positively correlated with disease activity.
CCL2及其受体CCR2在一系列炎症性疾病中的重要作用已有报道。然而，很少有研究探讨CCL2/CCR2轴在口腔扁平苔藓（OLP）中的潜在作用。因此，本研究旨在检测CCL2和CCR2在OLP病变中的表达，并比较治疗前后它们的变化。
采用免疫组织化学染色和实时逆转录聚合酶链反应（RT-PCR）检测32例OLP患者和8例对照者的CCL2和CCR2表达。此外，评估了3例患者病变部位经曲安奈德治疗后其表达的变化。
对照组中CCL2+和CCR2+细胞较少，而在病变的上皮层和上皮下层显著增加（n = 32，所有P < 0.001）。然而，网状病变（n = 12）和红斑/糜烂性病变（n = 20）之间CCL2+和CCR2+细胞密度无显著差异，尽管治疗后它们显著降低（分别为627.7 ± 108.2对258.3 ± 148.3，P = 0.017；1034.7 ± 74.6对648 ± 77.6，P = 0.003）。CCL2+/CCR2+细胞数量与疾病活动度呈正相关（相关系数分别为0.588；P < 0.001；0.409；P = 分别为0.02）。
本研究结果表明CCL2-CCR2轴参与OLP的发病机制，并与疾病活动度呈正相关。
