Ding Wona, Lin Li, Zhang Botao, Xiang Xianbo, Wu Jing, Pan Zhichong, Zhu Shihua
College of Science and Technology, Ningbo University, Ningbo, 315211, People's Republic of China.
Planta. 2015 Jul;242(1):203-13. doi: 10.1007/s00425-015-2296-2. Epub 2015 Apr 19.
The involvement of OsKASI in FA synthesis is found to play a critical role in root development of rice. The root system plays important roles in plant nutrient and water acquisition. However, mechanisms of root development and molecular regulation in rice are still poorly understood. Here, we characterized a rice (Oryza sativa L.) mutant with shortened roots due to a defect in cell elongation. Map-based cloning revealed that the mutation occurred in a putative 3-oxoacyl-synthase, an ortholog of β-ketoacyl-[acyl carrier protein] synthase I (KASI) in Arabidopsis, thus designated as OsKASI. OsKASI was found to be ubiquitously expressed in various tissues throughout the plant and OsKASI protein was localized in the plastid. In addition, OsKASI deficiency resulted in reduced fertility and a remarkable change in fatty acid (FA) composition and contents in roots and seeds. Our results demonstrate that involvement of OsKASI in FA synthesis is required for root development in rice.
研究发现，水稻中参与脂肪酸（FA）合成的OsKASI在水稻根系发育中起着关键作用。根系在植物获取养分和水分方面发挥着重要作用。然而，水稻根系发育及其分子调控机制仍知之甚少。在此，我们鉴定了一个水稻（Oryza sativa L.）突变体，该突变体由于细胞伸长缺陷而导致根短。图位克隆表明，该突变发生在一个假定的3-氧代酰基合成酶上，它是拟南芥中β-酮酰基-[酰基载体蛋白]合成酶I（KASI）的直系同源物，因此命名为OsKASI。研究发现OsKASI在植物的各个组织中普遍表达，且OsKASI蛋白定位于质体中。此外，OsKASI缺陷导致育性降低，以及根和种子中脂肪酸（FA）组成和含量发生显著变化。我们的结果表明，水稻根系发育需要OsKASI参与FA合成。
