Wang HongWei
School of Life Sciences, Tsinghua University, Beijing, 100084, China,
Sci China Life Sci. 2015 Aug;58(8):750-6. doi: 10.1007/s11427-015-4851-2. Epub 2015 Apr 19.
Recently, significant technical breakthroughs in both hardware equipment and software algorithms have enabled cryo-electron microscopy (cryo-EM) to become one of the most important techniques in biological structural analysis. The technical aspects of cryo-EM define its unique advantages and the direction of development. As a rapidly emerging field, cryo-EM has benefitted from highly interdisciplinary research efforts. Here we review the current status of cryo-EM in the context of structural biology and discuss the technical challenges. It may eventually merge structural and cell biology at multiple scales.
最近，硬件设备和软件算法方面的重大技术突破使冷冻电子显微镜（cryo-EM）成为生物结构分析中最重要的技术之一。冷冻电子显微镜的技术层面决定了其独特优势和发展方向。作为一个迅速兴起的领域，冷冻电子显微镜受益于高度跨学科的研究工作。在此，我们在结构生物学的背景下回顾冷冻电子显微镜的现状，并讨论技术挑战。它最终可能会在多个尺度上融合结构生物学和细胞生物学。
