Li Shimei, Zhang Ying, Niu Lichun, Wang Qianyun, Lu Xiaojuan, Zhao Xuejing, Liang Jing, Liu Huiling
The Department of Obstetrics and Gynecology, Chinese People's Liberation Army 323 Hospital, No.6, Jianshe West Road, Xi'an, 710054, Shaanxi, China.
Arch Gynecol Obstet. 2015 Nov;292(5):1075-81. doi: 10.1007/s00404-015-3695-0. Epub 2015 Apr 17.
Our aim was to study the role of AK000953 silencing for the killing effect of danazol on uterine fibroids.
Quantitative PCR was applied to identify differential expression of AK000953 in uterine fibroid tissue and normal uterine tissue. Then we isolated and cultured uterine fibroid cells, designed the siRNA of AK000953 to silence its expression in uterine fibroid cells, and detected the treatment effect of danazol and AK000953 siRNA on cell proliferation, cell apoptosis, and cell invasion. Finally, guinea pig model of uterine fibroids was constructed to verify the effect of AK000953 silencing on uterine fibroid treatment with danazol in vivo.
Quantitative PCR showed that the AK000953 gene was highly expressed in uterine fibroid tissue compared with normal uterine tissue (2.1 ± 0.15 vs. 0.8 ± 0.05, p < 0.01). After AK000953 silencing in uterine fibroid cells, we discovered that the inhibition rate in danazol-siRNA group was 56 ± 5 %, the cell apoptosis rate of danazol-siRNA group was 43 ± 2.3 %, and the invasion rate of uterine fibroid cells was 12 ± 1 %, which all showed significant differences with the control group or danazol group. Guinea pig model confirmed that the treatment of danazol and AK000953 siRNA effectively inhibited the development of fibroids in vivo.
AK000953 silencing could effectively enhance the killing effect of danazol on uterine fibroid cells.
我们的目的是研究AK000953基因沉默对达那唑治疗子宫肌瘤杀伤作用的影响。
采用定量PCR法检测子宫肌瘤组织和正常子宫组织中AK000953基因的差异表达。然后分离培养子宫肌瘤细胞，设计AK000953的小干扰RNA（siRNA）以沉默其在子宫肌瘤细胞中的表达，并检测达那唑和AK000953 siRNA对细胞增殖、细胞凋亡和细胞侵袭的治疗效果。最后构建豚鼠子宫肌瘤模型，在体内验证AK000953基因沉默对达那唑治疗子宫肌瘤的作用。
定量PCR结果显示，与正常子宫组织相比，AK000953基因在子宫肌瘤组织中高表达（2.1±0.15 vs. 0.8±0.05，p<0.01）。在子宫肌瘤细胞中沉默AK000953基因后，我们发现达那唑-siRNA组的抑制率为56±5%，达那唑-siRNA组的细胞凋亡率为43±2.3%，子宫肌瘤细胞的侵袭率为12±1%，与对照组或达那唑组相比均有显著差异。豚鼠模型证实，达那唑和AK000953 siRNA治疗可有效抑制体内肌瘤的生长。
AK000953基因沉默可有效增强达那唑对子宫肌瘤细胞的杀伤作用。
