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碳酸酐酶II作为构建生物相容性人工复分解酶的宿主蛋白。

Carbonic anhydrase II as host protein for the creation of a biocompatible artificial metathesase.

作者信息

Zhao Jingming, Kajetanowicz Anna, Ward Thomas R

机构信息

Department of Chemistry, University of Basel, Spitalstrasse 51, CH-4056 Basel, Switzerland.

出版信息

Org Biomol Chem. 2015 May 28;13(20):5652-5. doi: 10.1039/c5ob00428d. Epub 2015 Apr 20.

DOI:10.1039/c5ob00428d
PMID:25894419
Abstract

An artificial metathesase results from incorporation of an Hoveyda-Grubbs catalyst bearing an arylsulfonamide anchor within human carbonic anhydrase II. The optimization of the catalytic performance is achieved upon combining both chemical and genetic means. Up to 28 TONs were obtained within four hours under aerobic physiological conditions.

摘要

一种人工复分解酶是通过将带有芳基磺酰胺锚定基团的霍维达-格鲁布斯催化剂引入人碳酸酐酶II中产生的。通过化学和基因手段相结合实现了催化性能的优化。在有氧生理条件下，四小时内可获得高达28的催化转化数。

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