Wang Junming, Miao Mingsan, Zhang Yueyue, Liu Ruixin, Li Xaobing, Cui Ying, Qu Lingbo
Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China., College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China.
Can J Physiol Pharmacol. 2015 Jun;93(6):427-33. doi: 10.1139/cjpp-2015-0038. Epub 2015 Apr 20.
Quercetin (Que) is one of main compounds in Lysimachia christinae Hance (Christina loosestrife), and has both medicinal and nutritional value. Glycosides from Tripterygium wilfordii Hook.f. (léi gōng téng [the thunder duke vine]; TG) have diverse and broad bioactivities but with a high incidence of liver injury. Our previous study reported on the hepatoprotective properties of an ethanol extract from L. christinae against TG-induced liver injury in mice. This research is designed to observe, for the first time, the possible protective properties of the compound Que against TG-induced liver injury, and the underlying mechanisms that are involved in oxidative stress and anti-inflammation. The results indicated that TG caused excessive elevation in serum levels of alanine/aspartate transaminase (ALT/AST), alkaline phosphatase (ALP), gamma glutamyl transferase (γ-GT), and pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-α), as well as hepatic lipid peroxidation (all P < 0.01). On the other hand, following TG exposure, we observed significantly reduced levels of biomarkers, including hepatic glutathione (GSH), glutathione-S-transferase (GST), glutathione peroxidase (GPx), and the anti-inflammatory cytokine interleukin (IL)-10, as well as the enzyme activity and mRNA expression of copper- and zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) (all P < 0.01). Nevertheless, all of these alterations were reversed by the pre-administration of Que or the drug bifendate (positive control) for 7 consecutive days. Therefore, this study suggests that Que ameliorates TG-induced acute liver injury, probably through its ability to reduce oxidative stress and its anti-inflammatory properties.
槲皮素（Que）是金钱草中的主要化合物之一，具有药用和营养价值。雷公藤（TG）中的糖苷具有多样且广泛的生物活性，但肝损伤发生率较高。我们之前的研究报道了金钱草乙醇提取物对小鼠TG诱导的肝损伤的保肝特性。本研究旨在首次观察化合物Que对TG诱导的肝损伤的可能保护特性，以及其在氧化应激和抗炎方面的潜在机制。结果表明，TG导致血清丙氨酸/天冬氨酸转氨酶（ALT/AST）、碱性磷酸酶（ALP）、γ-谷氨酰转移酶（γ-GT）和促炎细胞因子肿瘤坏死因子-α（TNF-α）水平过度升高，以及肝脏脂质过氧化（所有P<0.01）。另一方面，在TG暴露后，我们观察到包括肝脏谷胱甘肽（GSH）、谷胱甘肽-S-转移酶（GST）、谷胱甘肽过氧化物酶（GPx）和抗炎细胞因子白细胞介素（IL）-10在内的生物标志物水平显著降低，以及含铜和锌的超氧化物歧化酶（CuZn-SOD）和过氧化氢酶（CAT）的酶活性和mRNA表达降低（所有P<0.01）。然而，连续7天预先给予Que或药物联苯双酯（阳性对照）可逆转所有这些改变。因此，本研究表明Que可能通过降低氧化应激和抗炎特性来改善TG诱导的急性肝损伤。
