Gomez-Nicola Diego, Riecken Kristoffer, Perry V Hugh, Fehse Boris
1 Centre for Biological Sciences, University of Southampton , Southampton SO166YD, United Kingdom .
2 Research Department Cell and Gene Therapy, Department of Stem Cell Transplantation, University Medical Centre Hamburg-Eppendorf , 20246 Hamburg, Germany .
Hum Gene Ther. 2015 Jun;26(6):332-3. doi: 10.1089/hum.2015.010. Epub 2015 Apr 20.
Identifying and tracking cells in the brain is a challenging task, requiring advanced molecular biology techniques and precise anatomical information. We have applied the principle of RGB marking to the long-term marking and tracking of progenitor and mature cells in the adult brain. Briefly, multicolor RGB marking is based on the simultaneous, lentiviral vector-mediated expression of three genes encoding fluorescent proteins in the three basic colors (red, green, and blue). Here, we show the application of RGB marking to the stable multicolor marking of adult granule cells in the hippocampus. This technique provides each individual cell with a characteristic hue, which facilitates their anatomical identification and spatial tracking. Multicolor RBG marking of mature neurons can provide an effective approach to dissect the function of individual cells, as it allows combination with functional techniques, facilitating the understanding of complex neuronal populations such as that of the dentate gyrus.
识别和追踪大脑中的细胞是一项具有挑战性的任务，需要先进的分子生物学技术和精确的解剖学信息。我们已将RGB标记原理应用于成体大脑中祖细胞和成熟细胞的长期标记与追踪。简而言之，多色RGB标记基于三种编码荧光蛋白的基因通过慢病毒载体介导在三种基本颜色（红色、绿色和蓝色）中同时表达。在此，我们展示了RGB标记在海马体成体颗粒细胞稳定多色标记中的应用。该技术为每个单独的细胞赋予一种特征色调，便于对其进行解剖学识别和空间追踪。成熟神经元的多色RBG标记可为剖析单个细胞的功能提供一种有效方法，因为它允许与功能技术相结合，有助于理解诸如齿状回等复杂神经元群体。
