Khalil Atef Mohammed, Yasuda Masahiro, Farid Ayman Samir, Desouky Mohamed Ibrahim, Mohi-Eldin Mouchira Mohammed, Haridy Mohie, Horii Yoichiro
Laboratory of Veterinary Parasitic Diseases, Faculty of Agriculture, University of Miyazaki, Gakuen-Kibanadai-Nishi 1-1, Miyazaki, 889-2192, Japan.
Parasitol Res. 2015 Jul;114(7):2735-42. doi: 10.1007/s00436-015-4480-5. Epub 2015 Apr 18.
We investigated the immunomodulatory and parasiticidal effects of garlic extract on coccidiosis caused by Eimeria vermiformis infection in male ICR mice. One group received garlic extract daily until the end of the experiment by the oral route from 10 days prior to oral infection with 300 sporulated E. vermiformis oocysts (infected-garlic(+)). The other group served as a control positive with E. vermiformis infection alone (infected-garlic(-)). In the infected-garlic(+) group, garlic extract treatment induced a significant reduction in fecal oocyst output when compared with the infected-garlic(-) group. Histopathological, immunohistochemical, and gene expression analysis for inflammatory cytokines in ileal tissues showed that the garlic extract treatment impaired intracellular development of E. vermiformis during the early stages by increasing the number of intraepithelial CD8(+) T cells and decreasing IL-10 expression. This induced cell cytotoxicity which was reflected by a decrease in oocyst numbers in the intestinal villi and the feces, indicating anticoccidial effects of the garlic extract. However, further studies to explore the precise mechanism of the observed effects of garlic treatment during Eimeria infection are needed to verify our results.
我们研究了大蒜提取物对雄性ICR小鼠由微小艾美耳球虫感染引起的球虫病的免疫调节和杀寄生虫作用。一组从口服感染300个孢子化微小艾美耳球虫卵囊前10天开始，每天经口给予大蒜提取物直至实验结束（感染大蒜组(+)）。另一组作为仅感染微小艾美耳球虫的对照阳性组（感染大蒜组(-)）。在感染大蒜组(+)中，与感染大蒜组(-)相比，大蒜提取物治疗导致粪便中卵囊排出量显著减少。对回肠组织中炎性细胞因子进行的组织病理学、免疫组织化学和基因表达分析表明，大蒜提取物治疗通过增加上皮内CD8(+) T细胞数量和降低IL-10表达，在早期阶段损害了微小艾美耳球虫的细胞内发育。这诱导了细胞毒性，表现为肠绒毛和粪便中卵囊数量减少，表明大蒜提取物具有抗球虫作用。然而，需要进一步研究以探索大蒜治疗在艾美耳球虫感染期间所观察到的作用的精确机制，以验证我们的结果。
