Gretskaia N M, Akimov M G, Bezuglov V V
Bioorg Khim. 2014 Mar-Apr;40(2):248-52.
For the first time a new fluorescent analogue of anadamide incorporating BODIPY®-FL-fluorophore, attached to arachidonic acid via 2,2'-(ethylenedioxy)-bis(ethylenediamine), was prepared. Using rat glioma C6 cells it was demonstrated that the fluorescent analogue is a substrate of the cellular anandamide uptake system (Km 4.5 ± 0.9 µM, Vmax 20 ± 1 amol/(min x cell)).
首次制备了一种新型的阿南达胺荧光类似物，该类似物包含通过2,2'-（乙二氧基）-双（乙二胺）连接到花生四烯酸上的BODIPY®-FL荧光团。利用大鼠胶质瘤C6细胞证明，该荧光类似物是细胞阿南达胺摄取系统的底物（Km为4.5±0.9μM，Vmax为20±1 amol/（min×细胞））。
