Siniakov A N, Nikolaenkova E B, Os'kina I A, Savel'ev V A, Samsonov V A, Tikhonov A Ia, Palatkina M Iu, Zaĭtsev D E
Bioorg Khim. 2014 Sep-Oct;40(5):636-8.
For the purpose of new detritylation agents search for microarray oligonucleotide synthesis we investigated applicability of 4-(4-methoxyphenyl)-2,6-dinitro-phenylmethyl 2,2,2-trichloroacetate for 4,4'-dimethoxytrityl group detritylation during oligonucleotide syntheses generating trichloroacetic acid at radiation by light with a length of wave of 405 nanometers. 4-(4-Methoxyphenyl)-2,6-dinitro-phenylmethyl 2,2,2-trichloroacetate has been successfully used for solid-phase oligonucleotide synthesis of desired oligonucleotides.
为了寻找用于微阵列寡核苷酸合成的新型去三苯甲基化试剂,我们研究了4-(4-甲氧基苯基)-2,6-二硝基苯基甲基2,2,2-三氯乙酸酯在寡核苷酸合成过程中对4,4'-二甲氧基三苯甲基基团进行去三苯甲基化的适用性,该合成过程在波长为405纳米的光辐射下会生成三氯乙酸。4-(4-甲氧基苯基)-2,6-二硝基苯基甲基2,2,2-三氯乙酸酯已成功用于所需寡核苷酸的固相寡核苷酸合成。