Yu Jia-jia, Cong Deng-li, Jiang Ying, Zhou Yuan, Wang Yan, Zhao Chun-fang
Zhong Yao Cai. 2014 Oct;37(10):1795-8.
To investigate the chemical constituents of Corydalis ochotensis and their antitumor bioactivity.
The compounds were isolated by silica gel column chromatography and recrystallization. Their structures were identified by spectroscopic analysis (NMR) and physicochemical properties. Their cytotoxic activity was studied by MTT.
Six compounds were elucidated as protopine (1), ochotensimine (2), fumariline (3), sanguinarine (4), tetrahydroberberine (5) and berberine (6). Compound 1 had excellent inhibitory activity on HepG2, SW480 and A549 cells, and compound 4 had excellent inhibitory activity on Hep2, HepG2, SW480 and A549 cells.
Compounds 3, 4 and 5 are isolated from this plant for the first time; In the MTT antitumor experiments,compounds 1 and 4 show an antitumor activity.
研究延胡索的化学成分及其抗肿瘤生物活性。
采用硅胶柱色谱法和重结晶法分离化合物。通过光谱分析（核磁共振）和理化性质鉴定其结构。采用MTT法研究其细胞毒性活性。
鉴定出6种化合物，分别为原阿片碱（1）、延胡索胺碱（2）、富马原碱（3）、血根碱（4）、四氢小檗碱（5）和小檗碱（6）。化合物1对HepG2、SW480和A549细胞具有优异的抑制活性，化合物4对Hep2、HepG2、SW480和A549细胞具有优异的抑制活性。
化合物3、4和5首次从该植物中分离得到；在MTT抗肿瘤实验中，化合物1和4显示出抗肿瘤活性。
