School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, China.
Development and Utilization Key Laboratory of Northeast Plant Materials Key Laboratory of Northeast Authentic Materials Research and Development in Liaoning Province School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, China.
Nat Prod Res. 2020 Dec;34(23):3305-3312. doi: 10.1080/14786419.2019.1566821. Epub 2019 Mar 25.
Two new alkaloids, including one benzophenanthridine type, dehydroambiguanine A and one isoquinoline type, ambiguanine J () together with four (-) known alkaloids were isolated from the -butanol fraction of subsp. . Their structures were elucidated by comprehensive spectroscopic methods including HR-ESIMS and NMR, and the absolute configuration of compound was further confirmed by the quantum ECD calculations. In addition, all isolated alkaloids () were determined their anti-proliferative effects of on A549 and HCT 116 cell by colorimetric MTT assay. Among them, Compounds and exhibited anti-proliferative activity on HCT 116 cell line with the IC value at 49.8 ± 4.79, and 89.2 ± 1.86 M, respectively. Compounds and showed mild anti-proliferative activity against A549 cell with IC values at 60.2 ± 10.7, and 89.2 ± 12.9 M, respectively. Their preliminary structure-activity relationship was also discussed.
从 subsp. 的正丁醇部分分离得到两个新的生物碱，包括一个苯并菲啶型的去氢ambiguanine A 和一个异喹啉型的ambiguanine J（），以及四个已知的生物碱（）。通过包括高分辨 ESIMS 和 NMR 在内的综合光谱方法阐明了它们的结构，并且通过量子 ECD 计算进一步确定了化合物的绝对构型。此外，通过比色 MTT 测定法确定了所有分离得到的生物碱（）对 A549 和 HCT 116 细胞的增殖抑制作用。其中，化合物和对 HCT 116 细胞系表现出增殖抑制活性，IC 值分别为 49.8±4.79 和 89.2±1.86 μM。化合物和对 A549 细胞表现出温和的增殖抑制活性，IC 值分别为 60.2±10.7 和 89.2±12.9 μM。还讨论了它们的初步构效关系。
