Ariotti Carla, Wagner Vivian Petersen, Salvadori Gabriela, Carrard Vinicius Coelho, Martins Marco Antônio Trevizani, da Cunha Filho Joao Julio, Meurer Luise, Martins Manoela Domingues
Department of Oral Pathology, School of Dentistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Institute of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway.
Tumour Biol. 2015 Sep;36(9):7285-92. doi: 10.1007/s13277-015-3454-7. Epub 2015 Apr 21.
The aim of the present study was to determine the role of vascular endothelial growth factor receptors (VEGFR1 and VEGFR2) in lip carcinogenesis, to investigate correlations between these markers with microvessel density (MVD) and clinicopathological aspects. Medical records from 27 cases of actinic cheilitis (AC) and 46 cases of lower lip squamous cell carcinoma (LLSCC) were analysed and submitted to immunohistochemistry. VEGFR1- and VEGFR2-immunostained sections were analysed based on percentage of positive epithelial and inflammatory cells, while CD31 was submitted to quantitative analysis to determine MVD. Different patterns of VGFR1 and VEGFR2 expression were observed between AC and LLSCC. VEGFR1 expression in epithelial and inflammatory cells and VEGFR2 expression in epithelial cells were higher in AC compared to LLSCC (p < 0.05). VEGFR1 expression in epithelial cells was higher in LLSCC compared to AC (p < 0.001). Expression of both receptors was not associated to MVD or clinicopathological aspects. A direct correlation was found between epithelial VEGFR1 and VEGFR2 expression (p = 0.02) and between VEGFR2 epithelial and inflammatory expression (p < 0.001). Our findings indicate that activation of VEGFR1 and VEGFR2 in epithelial and inflammatory cells appears to be an early event in lip carcinogenesis.
本研究的目的是确定血管内皮生长因子受体（VEGFR1和VEGFR2）在唇部癌变中的作用，研究这些标志物与微血管密度（MVD）及临床病理特征之间的相关性。分析了27例光化性唇炎（AC）和46例下唇鳞状细胞癌（LLSCC）的病历，并进行免疫组织化学检测。基于阳性上皮细胞和炎性细胞的百分比分析VEGFR1和VEGFR2免疫染色切片，而对CD31进行定量分析以确定MVD。在AC和LLSCC之间观察到VEGFR1和VEGFR2的不同表达模式。与LLSCC相比，AC中上皮细胞和炎性细胞中的VEGFR1表达以及上皮细胞中的VEGFR2表达更高（p < 0.05）。与AC相比，LLSCC中上皮细胞中的VEGFR1表达更高（p < 0.001）。两种受体的表达均与MVD或临床病理特征无关。上皮VEGFR1和VEGFR2表达之间（p = 0.02）以及VEGFR2上皮和炎性表达之间（p < 0.001）存在直接相关性。我们的研究结果表明，上皮细胞和炎性细胞中VEGFR1和VEGFR2的激活似乎是唇部癌变的早期事件。
