Salvadori Gabriela, Dos Santos Jean Nunes, Martins Marco Antonio Trevizani, Vasconcelos Artur Cunha, Meurer Luise, Rados Pantelis Varvaki, Carrard Vinicius Coelho, Martins Manoela Domingues
Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Tumour Biol. 2014 Aug;35(8):7635-44. doi: 10.1007/s13277-014-1989-7. Epub 2014 May 6.
Epithelial changes observed in actinic cheilitis (AC) and lower lip squamous cell carcinoma (LLSCC) have been studied using different markers in order to observe diagnostic and prognostic factors for both lesions. The aim of the present study was to analyze Ki-67, TGF-β1, and elastin content in AC and LLSCC to determine the possible role of these proteins in lip carcinogenesis. Medical records of 29 cases of AC and 53 cases of LLSCC were analyzed. Lesions were classified according histological pattern and submitted to immunostaining for Ki-67, TGF-β1, and elastin. Different percentages of Ki-67-positive cells were found in AC depending on the degree of epithelial dysplasia (p < 0.01). An association was also found between the percentage of Ki-67-positive cells and tumor grade in LLSCC (p < 0.01). An inverse correlation was found between Ki-67 and TGF-β1 in AC and LLSCC (p < 0.01). Elastosis was thinner and more discontinuous in LLSCC in comparison to AC, and this difference in the elastin immunolabeling pattern was statistically significant between groups (p < 0.01). The present findings indicate that changes in Ki-67 and TGF-β1 content contribute to lip carcinogenesis. Furthermore, elastin content reflects changes in the extracellular matrix in both AC and LLSCC.
为了观察光化性唇炎（AC）和下唇鳞状细胞癌（LLSCC）的诊断和预后因素，已使用不同标志物对这两种病变中观察到的上皮变化进行了研究。本研究的目的是分析AC和LLSCC中Ki-67、转化生长因子-β1（TGF-β1）和弹性蛋白的含量，以确定这些蛋白质在唇部致癌过程中的可能作用。分析了29例AC和53例LLSCC的病历。根据组织学模式对病变进行分类，并进行Ki-67、TGF-β1和弹性蛋白的免疫染色。根据上皮发育异常的程度，在AC中发现了不同百分比的Ki-67阳性细胞（p < 0.01）。在LLSCC中，还发现Ki-67阳性细胞百分比与肿瘤分级之间存在关联（p < 0.01）。在AC和LLSCC中，Ki-67与TGF-β1之间呈负相关（p < 0.01）。与AC相比，LLSCC中的弹性组织变性更薄且更不连续，并且弹性蛋白免疫标记模式的这种差异在组间具有统计学意义（p < 0.01）。目前的研究结果表明，Ki-67和TGF-β1含量的变化有助于唇部致癌。此外，弹性蛋白含量反映了AC和LLSCC中细胞外基质的变化。
