Karim A C, Woltgens J H, Bervoets T J, Lyaruu D M, Bronckers A L
Department of Anatomy, University of Manitoba, Winnipeg, Canada.
Anat Rec. 1989 Dec;225(4):318-28. doi: 10.1002/ar.1092250408.
The effect of adriamycin (1 mg/liter) on the development of the golden hamster 3-day-old second maxillary molars (M2) was investigated in vitro. Exposure of the molars to 1 mg/liter adriamycin during the first 2 hours of culture produced smaller teeth 3-7 days later, as determined by measurements of dry weights and by histological observations. Higher doses caused severe necrosis. The more differentiated pulp fibroblasts showed osteodentin formation 3 days after treatment with adriamycin (1 mg/liter), while the more immature ones underwent necrosis. The phenotypic changes brought on by the drug were permanent, and osteodentin continued to be formed throughout the course of this study. In addition the cervical loop region was inhibited from growing, while the production of the matrices of enamel and dentin appeared to be increased at 3 and 5 days after treatment. Electron microscopy of the forming osteodentin matrix revealed a random arrangement of banded collagen fibers during the early stage of osteodentin formation. As more matrix was formed, the collagen became quite compact and appeared quite similar to dentin. Finally, matrix vesicles were found among the collagenous matrix that was not yet mineralized. With the exception of the increased production of enamel and dentin, these in vitro results confirmed those earlier in vivo studies on the effect of adriamycin on rat incisor tooth.
研究了阿霉素（1毫克/升）对金黄仓鼠3日龄第二上颌磨牙（M2）体外发育的影响。在培养的最初2小时将磨牙暴露于1毫克/升阿霉素中，3 - 7天后牙齿变小，这通过干重测量和组织学观察得以确定。更高剂量会导致严重坏死。在用阿霉素（1毫克/升）处理3天后，分化程度更高的牙髓成纤维细胞显示出骨样牙本质形成，而更不成熟的细胞则发生坏死。药物引起的表型变化是永久性的，在本研究过程中骨样牙本质持续形成。此外，颈环区域的生长受到抑制，而在处理后3天和5天，釉质和牙本质基质的产生似乎增加。对正在形成的骨样牙本质基质的电子显微镜观察显示，在骨样牙本质形成的早期，带状胶原纤维呈随机排列。随着更多基质形成，胶原变得相当致密，并且看起来与牙本质非常相似。最后，在尚未矿化的胶原基质中发现了基质小泡。除了釉质和牙本质产生增加外，这些体外研究结果证实了早期关于阿霉素对大鼠切牙影响的体内研究结果。
