Karim A C, Wöltgens J H, Bervoets T J, Lyaruu D M, Bronchers A L
Department of Anatomy and Oral Biology, Faculty of Dentistry, University of Manitoba, Canada.
Exp Toxicol Pathol. 1993 May;45(2-3):113-20. doi: 10.1016/S0940-2993(11)80482-3.
The effect of a 2 hour exposure to adriamycin (1 mg/litre) on alkaline phosphatase (ALPase) activity of the golden hamster 4-5 day old second maxillary molars (M2) was investigated in vitro. The molars were grown in BGJb medium containing 15% fetal bovine serum, glutamine (200 micrograms/ml), vitamin C (250 micrograms/ml), penicillin G (50 micrograms/ml), and streptomycin sulphate (30 micrograms/ml). The gas phase contained 50% O2 + 5% CO2 + 45% N2. The molars were supported on cellulosic membrane filters and grown for 3, 5, and 7 days at the medium-gas interface in a closed humidified chamber. Biochemical analysis indicated a steady increase in ALPase activity throughout this study in the control samples. However, after adriamycin treatment no increase in ALPase activity could be observed. The histochemical data showed that the increased activity in the control was confined to the peripheral pulp, sub-odontoblastic layer, stratum intermedium, ameloblasts and odontoblasts. Although these layers showed a decreased activity after adriamycin treatment, the ameloblasts showed an increase in activity over the control. The data has shown that adriamycin caused a reduction in total ALPase activity in developing molars in vitro; osteodentin production by pulp cells; and appeared to produce an acceleration in the differentiation of ameloblasts.
体外研究了阿霉素（1毫克/升）2小时暴露对金黄仓鼠4 - 5日龄第二上颌磨牙（M2）碱性磷酸酶（ALPase）活性的影响。磨牙在含有15%胎牛血清、谷氨酰胺（200微克/毫升）、维生素C（250微克/毫升）、青霉素G（50微克/毫升）和硫酸链霉素（30微克/毫升）的BGJb培养基中生长。气相含有50% O2 + 5% CO2 + 45% N2。磨牙支撑在纤维素膜滤器上，在封闭的湿润培养箱中的培养基 - 气体界面处培养3、5和7天。生化分析表明，在整个研究过程中，对照样品中的ALPase活性稳步增加。然而，阿霉素处理后未观察到ALPase活性增加。组织化学数据显示，对照中活性增加局限于外周牙髓、成牙本质细胞下层、中间层、成釉细胞和成牙本质细胞。虽然这些层在阿霉素处理后活性降低，但成釉细胞的活性比对照增加。数据表明，阿霉素导致体外发育磨牙中总ALPase活性降低；牙髓细胞产生骨样牙本质；并且似乎加速了成釉细胞的分化。
