Mousavi Ghafour, Mohajeri Daryoush
Department of Clinical Sciences, College of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
Department of Pathobiology, College of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
Iran J Basic Med Sci. 2014 Dec;17(12):1032-5.
The aim of this study was to evaluate the effects of ground black seeds on renal tubular cell apoptosis following ischemia/reperfusion (I/R) injury in rats.
Forty male Wistar rats were randomly allocated into 5 equal groups including Sham, I/R model and three I/R+ black seeds (5, 10 and 20%)-treated groups. I/R groups' kidneys were subjected to 60 min of ischemia followed by 24 h of reperfusion. Microscopically, apoptosis of tubular cells was assessed by terminal deoxynucleotidyltransferase-mediated dUTP nick-end labelling (TUNEL) method.
The apoptotic cells of renal tubules were increased significantly (P<0.01) in I/R group than those in sham operation group. The TUNEL positive cells in black seeds (10% and 20%) treatment groups decreased significantly (P<0.05).
Inhibition of apoptosis may be responsible for the protective effects of black seeds in rats with renal I/R injury.
本研究旨在评估黑种草籽对大鼠缺血/再灌注（I/R）损伤后肾小管细胞凋亡的影响。
40只雄性Wistar大鼠被随机分为5组，每组数量相等，包括假手术组、I/R模型组和三个I/R+黑种草籽（5%、10%和20%）治疗组。I/R组的肾脏经历60分钟缺血，随后再灌注24小时。通过末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）法在显微镜下评估肾小管细胞的凋亡情况。
I/R组肾小管的凋亡细胞比假手术组显著增加（P<0.01）。黑种草籽（10%和20%）治疗组的TUNEL阳性细胞显著减少（P<0.05）。
抑制细胞凋亡可能是黑种草籽对大鼠肾I/R损伤具有保护作用的原因。
