在人皮质模型中光遗传学诱导的癫痫样活动。
Optogenetic induced epileptiform activity in a model human cortex.
作者信息
Selvaraj Prashanth, Sleigh Jamie W, Kirsch Heidi E, Szeri Andrew J
机构信息
Department of Mechanical Engineering, University of California, Berkeley, 94720 CA USA.
Waikato Clinical School, University of Auckland, Hamilton, New Zealand.
出版信息
Springerplus. 2015 Apr 1;4:155. doi: 10.1186/s40064-015-0836-7. eCollection 2015.
BACKGROUND
Cortical stimulation plays an important role in the study of epileptic seizures. We present a numerical simulation of stimulation using optogenetic channels expressed by excitatory cells in a mean field model of the human cortex.
FINDINGS
Depolarising excitatory cells in a patch of model cortex using Channelrhodpsin-2 (ChR2) ion channels, we are able to hyper-excite a normally functioning cortex and mimic seizure activity. The temporal characteristics of optogenetic channels, and the ability to control the frequency of synchronous activity using these properties are also demonstrated.
CONCLUSIONS
Optogenetics is a powerful stimulation technique with high spatial, temporal and cell-type specificity, and would be invaluable in studying seizures and other brain disorders and functions.
背景
皮层刺激在癫痫发作研究中发挥着重要作用。我们在人类皮层平均场模型中，对由兴奋性细胞表达的光遗传学通道的刺激进行了数值模拟。
研究结果
利用通道视紫红质-2（ChR2）离子通道使模型皮层的一片区域中的兴奋性细胞去极化，我们能够过度激发正常运作的皮层并模拟癫痫活动。还展示了光遗传学通道的时间特性，以及利用这些特性控制同步活动频率的能力。
结论
光遗传学是一种强大的刺激技术，具有高空间、时间和细胞类型特异性，在研究癫痫发作及其他脑部疾病和功能方面将具有极高价值。
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