College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China; The Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Laboratory of Molecule-Based Materials, Anhui Key Laboratory of Chemo-Biosensing, Anhui Key Laboratory of Functional Molecular Solids, PR China.
College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China; The Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Laboratory of Molecule-Based Materials, Anhui Key Laboratory of Chemo-Biosensing, Anhui Key Laboratory of Functional Molecular Solids, PR China.
Biosens Bioelectron. 2015 Sep 15;71:137-142. doi: 10.1016/j.bios.2015.04.037. Epub 2015 Apr 14.
A new strategy for a composite film based electrochemical sensor was developed in this work. A layer of conductive film of poly(p-aminobenzene sulfonic acid) (pABSA) was electropolymerized onto glassy carbon electrode surface and exhibited a high electrocatalytic active for paracetamol (PR) redox. The subsequent formation of a layer of molecular imprinted polymer (MIP) film on pABSA modified electrode endowed the sensor with plentiful imprinted cavities for PR specific adsorption. The advantages of the composite film made the prepared sensor display high sensitivity and good selectivity for PR detection and recognition. Under the optimal conditions, the sensor could recognize PR from its interferents. A linear ranging from 5.0 × 10(-8) to 1.0 × 10(-4)mol/L for PR detection was obtained with a detection limit of 4.3 × 10(-8)mol/L. The sensor has been applied to analyze PR in tablets and human urine samples with satisfactory results. The simple, low cost, and efficient strategy reported here can be further used to prepare electrochemical sensors for other compounds recognition and detection.
本工作提出了一种基于复合膜的电化学传感器的新策略。在玻碳电极表面电聚合了一层聚对氨基苯磺酸（pABSA）的导电膜，对扑热息痛（PR）的氧化还原表现出高电催化活性。随后在 pABSA 修饰电极上形成一层分子印迹聚合物（MIP）膜，赋予传感器丰富的 PR 特异性吸附印迹空穴。复合膜的优势使制备的传感器对 PR 的检测和识别具有高灵敏度和良好的选择性。在最佳条件下，传感器能够从干扰物中识别出 PR。对于 PR 的检测，获得了从 5.0×10(-8)到 1.0×10(-4)mol/L 的线性范围，检测限为 4.3×10(-8)mol/L。该传感器已成功用于分析片剂和人尿液样品中的 PR，结果令人满意。这里报道的简单、低成本、高效的策略可以进一步用于制备用于其他化合物识别和检测的电化学传感器。
