Motaharian Ali, Motaharian Fatemeh, Abnous Khalil, Hosseini Mohammad Reza Milani, Hassanzadeh-Khayyat Mohammad
Research Laboratory of Real Samples Analysis, Faculty of Chemistry, Iran University of Science and Technology, Tehran, 1684613114, Iran.
Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Anal Bioanal Chem. 2016 Sep;408(24):6769-79. doi: 10.1007/s00216-016-9802-7. Epub 2016 Aug 6.
In this research, an electrochemical sensor based on molecularly imprinted polymer (MIP) nanoparticles for selective and sensitive determination of diazinon (DZN) pesticides was developed. The nanoparticles of diazinon imprinted polymer were synthesized by suspension polymerization and then used for modification of carbon paste electrode (CPE) composition in order to prepare the sensor. Cyclic voltammetry (CV) and square wave voltammetry (SWV) methods were applied for electrochemical measurements. The obtained results showed that the carbon paste electrode modified by MIP nanoparticles (nano-MIP-CP) has much higher adsorption ability for diazinon than the CPE based non-imprinted polymer nanoparticles (nano-NIP-CP). Under optimized extraction and analysis conditions, the proposed sensor exhibited excellent sensitivity (95.08 μA L μmol(-1)) for diazinon with two linear ranges of 2.5 × 10(-9) to 1.0 × 10(-7) mol L(-1) (R (2) = 0.9971) and 1.0 × 10(-7) to 2.0 × 10(-6) mol L(-1) (R (2) = 0.9832) and also a detection limit of 7.9 × 10(-10) mol.L(-1). The sensor was successfully applied for determination of diaznon in well water and apple fruit samples with recovery values in the range of 92.53-100.86 %. Graphical abstract Procedure for preparation of electrochemical sensor based on MIP nanoparticles for determination of diazinon.
在本研究中，开发了一种基于分子印迹聚合物（MIP）纳米颗粒的电化学传感器，用于选择性和灵敏地测定二嗪农（DZN）农药。通过悬浮聚合法合成了二嗪农印迹聚合物纳米颗粒，然后将其用于修饰碳糊电极（CPE）的组成以制备传感器。采用循环伏安法（CV）和方波伏安法（SWV）进行电化学测量。所得结果表明，用MIP纳米颗粒修饰的碳糊电极（nano-MIP-CP）对二嗪农的吸附能力比基于非印迹聚合物纳米颗粒的CPE（nano-NIP-CP）高得多。在优化的萃取和分析条件下，所提出的传感器对二嗪农表现出优异的灵敏度（95.08 μA L μmol⁻¹），线性范围为2.5×10⁻⁹至1.0×10⁻⁷ mol L⁻¹（R² = 0.9971）和1.0×10⁻⁷至2.0×10⁻⁶ mol L⁻¹（R² = 0.9832），检测限为7.9×10⁻¹⁰ mol·L⁻¹。该传感器成功应用于井水和苹果果实样品中二嗪农的测定，回收率在92.53 - 100.86%范围内。图形摘要基于MIP纳米颗粒的电化学传感器测定二嗪农的制备程序。
